NISCAIR Online Periodicals Repository Collection: IJEB Vol.46(04) [April 2008]

Book Review

Sat, 29 Mar 2008 22:58:59 GMT

Title: Book Review

Abstract: Microbial Biotechnology
G S Randhawa

Page(s): 258

Antimicrobial protein from Streptomyces fulvissimus inhibitory to methicillin resistant Staphylococcus aureus

Sat, 29 Mar 2008 22:58:59 GMT

Title: Antimicrobial protein from Streptomyces fulvissimus inhibitory to methicillin resistant Staphylococcus aureus

Authors: Malik, Himadri; Sur, Banani; Singhal, Neha; Bihari, Vinod

Abstract: Fermented culture of Streptomyces fulvissimus was found to secrete an antibacterial protein inhibitory to Micrococcus luteus, Bacillus subtilis, Bacillus cereus and methicillin resistant Staphylococcus aureus (MRSA) strains. The extracellular protein from the fermented culture on concentration revealed a high molecular weight peptide of 63kDa on SDS-PAGE gel and the region on gel displayed inhibitory activity against methicillin resistant Staphylococcus aureus. Bioactivity of the extra cellular protein was non-sensitive to proteinase K, ⍺ chymotrypsin, protease, EDTA (ethylene diamine tetra acetic acid), PMSF (phenyl methyl sulfonyl fluoride) and DMSO (dimethyl sulfoxide) but partially susceptible to amylase and heat. Glycoprotein nature of the proteinaceous compound was confirmed by periodic acid schiff’s (PAS) staining. The secretary protein of S. fulvissimus demonstrated a significant activity against MRSA strain. It could be an important source for developing new drugs to control multidrug resistant gram positive bacteria .

Page(s): 254-257

Screening for disease resistance in barley cultivars against Bipolaris sorokiniana using callus culture method

Sat, 29 Mar 2008 22:58:59 GMT

Title: Screening for disease resistance in barley cultivars against Bipolaris sorokiniana using callus culture method

Authors: Chand, Ramesh; Sen, Devyani; Prasad, K D; Singh, A K; Bashyal, B M; Prasad, L C; Joshi, A K

Abstract: Screening for resistant barley genotypes in response to fungal toxin of Bipolaris sorokiniana was assessed on standing barley plants as well as in selected callus lines of the same. For the standing lines tested, those manifesting chlorosis in response to toxin infiltration showed a significantly slower disease progress as compared to the necrotic lines. Also, necrosis in the callus tissues of the susceptible cultivar in MS medium supplemented with different concentrations of the crude toxin was significantly higher than in the callus tissues of the chlorotic lines studied. Similar host response to the toxin in in vitro and field situations open up the possibility of screening barley cultivars for resistance to spot blotch using callus culture as against classical methods of screening in order to increase accuracy and save time and space.

Page(s): 249-253

Effects of different factors on immature embryo culture, PLBs differentiation and rapid mass multiplication of Coelogyne suaveolens (Lindl.) Hook

Sat, 29 Mar 2008 22:58:59 GMT

Title: Effects of different factors on immature embryo culture, PLBs differentiation and rapid mass multiplication of Coelogyne suaveolens (Lindl.) Hook

Authors: long, Sungkum; Deb, Chitta Ranjan

Abstract: In vitro mass production of C. suaveolens (Lindl.) Hook, an endangered orchid with its snowy white flowers having horticultural potential was accomplished through immature seed culture, and subsequent plant regeneration. The developmental stage of the immature seeds and nutrient media significantly influenced the germination frequency. Seeds at 13 months after pollination cultured on 3% sucrose containing Murashige and Skoog (MS) medium with 9 µM a-naphthaleneacetic acid (NAA), and 15% coconut water exhibited 93% germination after 40 days of culture. Upon subculture, the germinated shoots on MS medium with 9 µM BA, 6 µM NAA, 3% casein hydrolysate and 0.1% activated charcoal (AC) yielded >12 shoots per shoot or bud. Addition of AC favoured the enlargement of pseudobulbs and better rooting. The plantlets transferred to community potting mix after in vitro hardening (8-10 wk) displayed 85% survival.

Page(s): 243-248