http://nopr.niscair.res.in/handle/123456789/3477 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/3646 Title: Measurement of urinary oxalate by grain sorghum leaf oxalate oxidase immobilized to affixed alkylamine glass beads <br/> <br/>Authors: Kumari, M; Pundir, C S <br/> <br/>Abstract: Oxalate in urine was measured by grain Sorghum leaf oxalate oxidase conjugated to alkyl amine glass beads affixed in a beaker. The minimum detection limit was 0.05 mM/L in urine. Recovery of added oxalate in urine was 80.5% and within and between assay, coefficients of variation (CV) were <4% and <5.5%, respectively. Urinary oxalate values obtained by the present method showed a good correlation (r = 0.947) with those by Sigma kit method. The method is not only free from tedious handling of free glass beads and Cl⁻ interference, but also has longer stability and reusability of immobilized enzyme compared to that of barley root and forage Sorghum leaf. <br/> <br/>Page(s): 102-106 Mon, 29 Mar 2004 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/3645 Title: Absorption spectral study of 3, 4-dihydroxy phenyl alanine (DOPA) with Nd (III) in aqueous medium <br/> <br/>Authors: Bhatt, Prashant N; Pathak, Trupti V; Mehta, Jignasu P; Misra, Sudhindra N <br/> <br/>Abstract: Spectroscopic properties of Nd (III)-DOPA in aquated organic solvents are investigated through absorption difference and comparative absorption spectroscopy involving 4f-4f transitions. The change in coordination in different conditions is observed from the absorption intensity analysis when Nd (III) interacts with DOPA. The interaction induced substantial changes in the intensities of 4f-4f bands and their perturbation was reflected through oscillator strength Judd-Ofelt intensity (T) parameters. It is suggested that the changes in the oscillator strengths of different 4f-4f bands and Judd-Ofelt intensity (T) parameters can be used to predict in vivo intracellular complexation of DOPA with Ca(II) through Nd (III)-DOPA absorption spectral studies in-vitro as both Nd (III) and Ca(II) have unique similarity in their coordination behaviour. <br/> <br/>Page(s): 123-127 Mon, 29 Mar 2004 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/3644 Title: Microwave-assisted method for microassay of proteins using bicinchoninic acid <br/> <br/>Authors: Jain, Sulakshana; Sharma, Shweta; Gupta, Munishwar N <br/> <br/>Abstract: A microwave-assisted microassay has been developed for estimation of proteins, using bicinchoninic acid (BCA). The protocol described here is rapid with a reaction time of merely 95 sec, requires lesser amounts of BCA and other reagents, and tolerates larger or comparable concentration of various interfering substances. <br/> <br/>Page(s): 113-115 Mon, 29 Mar 2004 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/3643 Title: Purification and characterization of an extracellular agglutinin from Tricophyton rubrum with specificity towards sialic acid containing glycoconjugates <br/> <br/>Authors: Bhowal, J; Mitra, A; Banerjee, S; Sikdar, S; Guha, A K; Chatterjee, B P <br/> <br/>Abstract: An agglutinin, a monomeric glycoprotein with a molecular mass of about 6.5 kDa and containing 18% sugar has been purified to an apparent homogeneity from a 21 days old culture filtrate of an anthropophilic dermatophyte Tricophyton rubrum. It is a human blood group non-specific agglutinin which also agglutinates animal erythrocytes and Ehrlich ascites carcinoma and Sarcoma-180 cells. It is thermally stable and exhibits maximum activity at pH 8. Amino acid analysis shows a significant amount of glycine, with no cysteine. Glycoproteins inhibited the hemagglutination of the agglutinin, but not the simple sugars, including sialic acid. Fetuin is the most potent inhibitor among the glycoproteins tested. This inhibition gives a hint to binding with Gal β1-3GalNAc or Gal β1-4GlcNAc residue containing sialic acid at the terminal position with α 2-6 or α 2-3 linkage. <br/> <br/>Page(s): 81-88 Mon, 29 Mar 2004 22:58:59 GMT