http://nopr.niscair.res.in/handle/123456789/3269 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/3290 Title: Correlation between biochemical properties and adaptive diversity of skeletal muscle myofibrils and myosin of some air-breathing teleosts <br/> <br/>Authors: Ahmad, Riaz; Hasnain, Absar-ul <br/> <br/>Abstract: Functional properties of myofibrils and relative stability of myosin of five teleosts Channa punctata, Clarias batrachus, Mastacembalus armatus, Labeo rohita and Catla catla adapted to different breathing modes were compared. Myofibrillar contractility and m-ATPase of air-breathing organ (ABO) possessing C. punctata and C. batrachus were low and least affected by pH in the range of 7.1-8.5. However, their myosin isoforms were relatively thermostable, more soluble at sub-neutral pH values, between 0.1 to 0.15 M KCl concentrations and less susceptible to α-chymotryptic digestion. In contrast, myofibrils and myosin of water-breather major carps L. rohita and C. catla were more contractile and susceptible to pH and salt concentrations. Thus, correlation between catalytic efficiency and relative stability of myofibrils and myosin of ABO-possessing teleosts was of reverse order and magnitude, as compared to water-breathers. Interestingly, myofibrils and myosin of the behavioral air-breather M. armatus showed intermediate properties. The specific levels of m-ATPase of all the five teleosts were in conformity with the levels of metabolic marker, the lactate dehydrogenase. The effect of chymotryptic cleavage of 94 and 173 kDa domains on ATPase, individuality of peptide maps of MyHC isomers and perturbation of phenylalanine residues by urea implicated hydrophobic residues in stabilizing myosin structure in these fish. The present study suggests two apparent evolutionary modifications of myofibrils and myosin in ABO-possessing teleosts: (i), ‘down-regulation’ of ATPase that explains sluggishness of such species and, (ii), more stable molecular structure to support stress of air-breathing modes of life. <br/> <br/>Page(s): 217-225 Sat, 29 Jul 2006 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/3289 Title: Preparation of prospective plant oil derived micro-emulsion vehicles for drug delivery <br/> <br/>Authors: Gupta, Syamasri; Sanyal, S K; Datta, S; Moulik, S P <br/> <br/>Abstract: Biocompatible oil-in-water (o/w) micro-emulsions can be prospective drug delivery vehicles for their capability to solubilize lipophilic (oil soluble) drugs in the dispersed oil. Plant oils are considered suitable for such a purpose. In this study, we have attempted to examine the dispersion of corn, cottonseed, clove, orange and peppermint oils, as well as isopropyl myristate (IPM) in water continuum in presence of surfactants Tween-20, Brij-30 and Brij-92 and co-surfactants ethanol (EtOH) and isopropyl alcohol (iPrOH). Both ternary (oil/surfactant/water) and psedo-ternary (oil/surfactant + co-surfactant/water) phase diagrams were constructed. The ternary systems produced larger micro-emulsion forming zones than the psedo-ternary systems. The combinations peppermint oil/iPrOH/water, IPM/iPrOH/water and 1:1 (v/v) peppermint oil + IPM/iPrOH/water were found to form fair proportion of single-phase surfactant-less micro-emulsion. The surfactant-aided ternary systems produced larger clear micro-emulsion zones, compared to pseudo-ternary systems, while the behaviour of surfactant-less systems was intermediate. The prepared systems had shelf life of 1 year and they withstood temperature variations in the range of 4-40°C. <br/> <br/>Page(s): 254-257 Sat, 29 Jul 2006 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/3287 Title: Nitric oxide levels during erythroid differentiation in K562 cell line <br/> <br/>Authors: Küçükkaya, B; Öztürk, G; Yalçıntepe, L <br/> <br/>Abstract: Nitric oxide (NO) is endogenous mediator of numerous physiological processes that range from regulation of cardiovascular function and neurotransmission to antipathogenic and tumoricidal responses. This study was designed to investigate the possible role of NO during erythroid differentiation in K562 erythroleukemia cells. The chronic myelogenous leukemia (K562) cell line can be triggered in culture to differentiate along the erythrocytic pathway, in response to a variety of stimulatory agents. In this study, K562 cells were induced to synthesize hemoglobin by hemin. We investigated NOx (nitrate+nitrite) levels in uninduced (control) and hemin-induced K562 cell lysates during erythroid differentiation. Our results showed that NO levels decreased significantly on fourth and sixth day both in hemin-induced and control cells; the decrease was, however, more in hemin-induced group than in control group. <br/> <br/>Page(s): 251-253 Sat, 29 Jul 2006 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/3286 Title: Isolation of stress responsive Psb A gene from rice (Oryza sativa L.) using differential display <br/> <br/>Authors: Tyagi, Aruna; Chandra, Arti <br/> <br/>Abstract: Differential display (DD) experiments were performed on drought-tolerant rice (Oryza sativa L.) genotype N22 to identify both upregulated and downregulated partial cDNAs with respect to moisture stress. DNA polymorphism was detected between drought-stressed and control leaf tissues on the DD gels. A partial cDNA showing differential expression, with respect to moisture stress was isolated from the gel. Northern blotting analysis was performed using this cDNA as a probe and it was observed that mRNA corresponding to this transcript was accumulated to high level in rice leaves under water deficit stress. At the DNA sequence level, the partial cDNA showed homology with psb A gene encoding for D1 protein. <br/> <br/>Page(s): 244-246 Sat, 29 Jul 2006 22:58:59 GMT