http://nopr.niscair.res.in/handle/123456789/2930 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/2963 Title: Recent advances in medicinal plant biotechnology <br/> <br/>Authors: Khan, Mohammad Yaseen; Aliabbas, Saleh; Kumar, Vimal; Rajkumar, Shalini <br/> <br/>Abstract: Medicinal plants are the most important source of life saving drugs for the majority of the world’s population. Plant secondary metabolites are economically important as drugs, fragrances, pigments, food additives and pesticides. The biotechnological tools are important to select, multiply, improve and analyze medicinal plants. In-vitro production of secondary metabolites in plant cell suspension cultures has been reported from various medicinal plants and bioreactors are the key step towards commercial production of secondary metabolites by plant biotechnology. Genetic transformation is a powerful tool for enhancing the productivity of novel secondary metabolites; especially by Agrobacterium tumefacians. Combinatorial biosynthesis is another approach in the generation of novel natural products and for the production of rare and expensive natural products. DNA profiling techniques like DNA microarrays serve as suitable high throughput tools for the simultaneous analysis of multiple genes and analysis of gene expression that becomes necessary for providing clues about regulatory mechanism, biochemical pathways and broader cellular functions. <br/> <br/>Page(s): 9-22 Mon, 29 Dec 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/2962 Title: Micropropagation of Wrightia tomentosa : Effect of gelling agents, carbon source and vessel type <br/> <br/>Authors: Joshi, Preeti; Trivedi, Rohini; Purohit, S D <br/> <br/>Abstract: The role of gelling agents, carbon source, type of culture vessel and liquid culture system during rooting phase in Wrightia tomentosa was investigated in an effort to reduce the cost of micropropagation. Studies have revealed significant improvement in shoot multiplication on a medium containing crude agar and sugarcubes. Promotory role of vented vessel in culture growth and liquid culture system during rooting phase was established. The results showed strong potential for reduction in cost of plantlets in vitro. <br/> <br/>Page(s): 115-120 Mon, 29 Dec 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/2961 Title: Characterization of thermoalkalophilic xylanase isolated from Enterobacter isolates <br/> <br/>Authors: Sharma, Anjana; Pujari, Rajesh; Patel, Pratibha <br/> <br/>Abstract: Thermoalkalophilic xylanase was isolated from Enterobacter spp. with significant activity. Maximum enzyme activity was observed in isolate BGCC#259 (E. cloacae), i.e., 0.056 IU/mL at 24 h, while the highest biomass was observed at 36 h of incubation. Hydrolysis study revealed complete degradation of xylan to xylose after 12 h of incubation. The molecular mass of the xylanase was ~43 kDa. The enzyme was characterized at varied range of pH and temperature, and it was found that all five Enterobacter isolates had a pH and temperature optima of 8.0 and 80ºC, respectively. At 80ºC, xylanase from isolate BGCC#254 (E. cloacae) retained 100% activity, while BGCC#259 retained more than 90% activity after 24 h. <br/> <br/>Page(s): 110-114 Mon, 29 Dec 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/2960 Title: Characterization of functional activity in composted casing amendments used in cultivation of Agaricus bisporus (Lange) Imbach <br/> <br/>Authors: Choudhary, Devendra K; Agarwal, Pavan K; Johri, Bhavdish N <br/> <br/>Abstract: In cultivation of button mushroom [Agaricus bisporus (Lange) Imbach], casing layer that is nutritionally deficient to compost is believed to trigger the fruit body formation and this is conducted by the bacterial community residing in casing layer. The change in nutritional status of the casing is highly correlated with microbial flora. Therefore, an attempt was made to characterize the bacterial flora in casing layer, i.e., Farm Yard Manure and Spent Mushroom Substrate (FYM+SMS, 3:1) and Farm Yard Manure and Vermi Compost (FYM+VC, 3:1), employing phenetic approaches. Morphotypically different and functionally characterized bacterial isolates were identified by partial 16S rDNA gene fragment sequencing. Available data showed a significant variety of organisms that included Acinetobacter and Pseudomonas of the γ-proteobacteria, that were most frequently encountered genera. Amongst Gram-positive bacteria, Bacillus was the most highly represented genus that was derived from the agaric fruit bodies. In addition, FYM+SMS was found to be a high yielding casing mixture, which took minimum case run period together with superior fruit body quality as compared to FYM+VC. <br/> <br/>Page(s): 97-109 Mon, 29 Dec 2008 22:58:59 GMT