http://nopr.niscair.res.in/handle/123456789/2863 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/2898 Title: Efficient in vitro plant regeneration via leaf base segments of indica rice (Oryza sativa L.) <br/> <br/>Authors: Ramesh, M; Murugiah, V; Gupta, Aditya K <br/> <br/>Abstract: A reliable and reproducible protocol has been developed for high frequency plant regeneration from 4-5 mm long leaf base segments of 4 days old in vitro germinated seedlings of indica rice (Oryza sativa) cultivar Rasi. The effect of age of seedlings, position of leaf base segments and optimum concentration of 2,4-D on callus induction frequency was investigated with a future aim to use leaf bases for biolistic and Agrobacterium-mediated transformation experiments. Friable, nodular and white to pale yellow embryogenic callus cultures (206 mg fresh weight /explant) were obtained from the first basal segments of rice seedlings on Linsmaier and Skoog (LS) medium supplemented with 2,4-D (11.3 μM) and 3.0 μM thiamine–HCL. Plant regeneration was achieved after the transfer of 54 days old embryogenic callus cultures to Murashige and Skoog (MS) medium supplemented with BAP (2.2 μM) and NAA (0.27 μM). In vitro regenerated plants with multiple shoots and roots transferred to sterile soil in growth chamber and maintained in greenhouse exhibited normal growth and were phenotypically similar to plants germinated from seeds. <br/> <br/>Page(s): 68-74 Mon, 29 Dec 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/2897 Title: Inhibition of Aspergillus flavus colonization and aflatoxin (AfB1) in peanut by methyleugenol <br/> <br/>Authors: Sudhakar, P; Latha, P; Sreenivasulu, Y; Reddy, B V Bhaskar; Hemalatha, T M <br/> <br/>Abstract: Methyleugenol is naturally occurring substance in oils and fruits and in various foods as flavoring agent. Effect of this methyleugenol in inhibiting A. flavus colonization and aflatoxin production on peanut pods and kernels has been studied. Spray of methyleugenol (0.5 %) on peanut pods and kernels checked the colonization of A. flavus and aflatoxin synthesis. This chemical can be used as both prophylactic or post infection spray on peanut pods before storage. It is the first report on the inhibition of A.flavus by methyleugenol on peanut. <br/> <br/>Page(s): 63-67 Mon, 29 Dec 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/2896 Title: Protective effect of Withaferin-A on tumour formation in 7,12-dimethylbenz[a]anthracene induced oral carcinogenesis in hamsters <br/> <br/>Authors: Manoharan, S; Panjamurthy, K; Menon, Venugopal P; Balakrishnan, S; Alias, Linsa Mary <br/> <br/>Abstract: With an aim to investigate the protective effect of Withaferin-A on 7,12-dimethylbenz[a]anthracene (DMBA) induced oral carcinogenesis in Syrian golden hamsters, tumour incidence, tumour volume and tumour burden and status of detoxication agents, lipid peroxidation and antioxidants in DMBA administered (3 times/week for 14 weeks) hamsters were assessed. Hundred percent tumour formation in DMBA alone administered animal was observed. Oral administration of Withaferin-A (20 mg/kg body weight) to DMBA administered animals for 14 weeks completely prevented the tumour incidence, tumour volume and tumour burden. Also, Withaferin-A showed significant anti-lipid peroxidative and antioxidant properties and maintained the status of phase-I and phase-II detoxication agents during DMBA-induced oral carcinogenesis. The results thus indicate that the protective effect of Withaferin-A is probably due to its anti-lipid peroxidative and antioxidant functions as well as modulating effect on carcinogen detoxication during DMBA-induced oral carcinogenesis. <br/> <br/>Page(s): 16-23 Mon, 29 Dec 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/2895 Title: Production of peptide antifungal antibiotic and biocontrol activity of Bacillus subtilis <br/> <br/>Authors: Kumar, Ajay; Saini, Pragati; Shrivastava, J N <br/> <br/>Abstract: Among different bacterial cultures, a potent Bacillus subtilis MTCC-8114 was isolated from garden soil samples which showed 16 and 14 mm inhibition zones by spot inoculation method and 24 and 22 mm inhibition zones by well agar diffusion method against test fungi i.e. Microsporum fulvum and Trichophyton species. Among four media tested, the maximum growth and antibiotic production was found in trypticase soya broth (TSB) medium at 37º C, pH-7 and 48 h of incubation. The Rf value (0.64) by Thin Layer Chromatography (TLC) technique and UV and FTIR spectral data of the active antifungal compound, indicated that the isolated compound belongs to peptide antifungal antibiotic group. MIC value of antifungal antibiotic was 135 and 145 μg/ml. <br/> <br/>Page(s): 57-62 Mon, 29 Dec 2008 22:58:59 GMT