http://nopr.niscair.res.in/handle/123456789/2743 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/2766 Title: Insight into bio-physiological functions of acid phosphatase from potatoes (Solanum tuberosum): A bioanalytical approach <br/> <br/>Authors: Rane, Sandhya Y; Badave, Kirti D; Ahmed, Khursheed <br/> <br/>Abstract: The molecular mass of acid phosphatase (APase) from Solanum tuberosum is estimated as 111 kDa using denaturing SDS-PAGE. The prime catalytic di-iron Fe²⁺/Fe³⁺ site (1.62 ± 0.2 mole) together with the co-catalytic sites of manganese, zinc and trace copper (0.397 ± 0.135 mole) in the enzyme have been determined by the GF-AAS technique. The quantitation of the biphasic release of iron species from the enzyme has been carried out spectrophotometrically. The reduction (~70-72%) in biological activity of APase after removal of the divalent metal constellation of co-catalytic site made up of Fe²⁺, Mn²⁺, Cu²⁺ and Zn²⁺ is established after duplicate treatment of enzyme from a Chelex-100 column. The significance of redox active Fe²⁺/Fe³⁺ metal cofactors at the active site of the enzyme correlates well with the affinity of substrate binding towards the di-iron site in terms of kinetic parameters. A physiological defense activity of APase is established in terms of its quantitative pUC-19 plasmid DNA cleavage activity. <br/> <br/>Page(s): 15-24 Mon, 29 Dec 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/2765 Title: Conductance studies on some alkali metal acetates in aqueous glycerol solutions <br/> <br/>Authors: Sarkar, Bipul Kumar; Roy, Mahendra Nath; Sinha, Biswajit <br/> <br/>Abstract: Molar conductance of lithium acetate, sodium acetate, potassium acetate, sodium bromide, sodium tetraphenylborate and tetrabutylammonium bromide in a series of glycerol + water mixtures at temperatures from 298.15 K to 318.15 K has been reported. The limiting polar conductances, association constants and cosphere diameter have been derived from the Lee-Wheaton conductivity equation. The limiting ionic conductivities have been calculated by reference electrolyte method. Ionic conductivities of cations and anions are discussed in terms of solvent viscosity, the Stokes radii and ion-ion and ion-solvent interactions. The small values of association constants for all the electrolytes studied indicate their weak association in aqueous glycerol mixtures, which is in line with the high dielectric constants of the solvent media. <br/> <br/>Page(s): 63-68 Mon, 29 Dec 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/2764 Title: Fabrication of Gd₂O₃：Eu³⁺ nanofibers by electrospinning <br/> <br/>Authors: Zhang, X Y; Chai, L Y; Liu, W <br/> <br/>Abstract: One-dimensional Gd₂O₃:Eu³⁺ nanofibers with cubic phase have been prepared as potential red phosphors by an electrospinning technique, and characterized by scanning electron microscopy, X-ray diffraction, FT-IR, TGA-DTA and photoluminescence spectra. The effect of annealing temperature on the crystallization and luminescence properties has been studied. The main emission position is centered at 611nm (⁵D₀ → ⁷F₂). Photoluminescence spectra indicate that fluorescence capability improves with increase in temperature. <br/> <br/>Page(s): 69-72 Mon, 29 Dec 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/2763 Title: A comparative study of partial molar volumes of some hydrated and anhydrous salts of transition metal sulphates and magnesium sulphate in water at different temperatures <br/> <br/>Authors: Parmar, M L; Sharma, Praveen; Guleria, M K <br/> <br/>Abstract: Partial molar volumes of some hydrated and anhydrous salts of transition metal sulphates, viz., cobalt sulphate, nickel sulphate copper sulphate, zinc sulphate and magnesium sulphate have been determined in water at five equidistant temperatures (298.15, 303.15, 308.15, 313.15 and 318.15 K). The density data have been analysed by means of Mason’s equation. The partial molar volumes ( ) and experimental slopes ( ) have been interpreted in terms of ion-solvent and ion-ion interactions, respectively. The partial molar volumes vary with temperature as a power series of temperature. Structure-making/breaking capacities of hydrated and anhydrous salts have been inferred from the sign of , i.e., the second derivative of partial molar volume with respect to temperature at constant pressure. The hydrated salts act as structure makers while anhydrous salts act as structure breakers in water, i.e., the behaviour is reversed on removal of water of crystallization. <br/> <br/>Page(s): 57-62 Mon, 29 Dec 2008 22:58:59 GMT