http://nopr.niscair.res.in/handle/123456789/2697 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/2711 Title: Polaron hopping in some biomolecular solids and their charge transfer complexes <br/> <br/>Authors: Solanki, G K; Amin, Anand; Padhiyar, Ashvin; Ray, A K; Oza, A T <br/> <br/>Abstract: The solid state spectroscopy of charge transfer complexes of biomolecules such as fatty acids, tripalmitin, lysozyme. folic acid, β-carotene, cytochrome c, valinomycin and gramicidin has been carried out. The absorption coefficient is related with electronic conductivity. A half-power beta density is found common among these macromolecular solids, indicating photon-induced polaron hopping or hopping of a charge carrier between two branches of a polariton. Band gap vs full width at half-maximum of the mid-IR peak also reveals a linear relation. <br/> <br/>Page(s): 421-429 Fri, 28 Nov 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/2710 Title: Semi-empirical calculations on paullones, a promising class of cyclin-dependent kinase inhibitors <br/> <br/>Authors: Sharma, Pooja Sapra; Tyagi, Reena; Sharma, Rajan <br/> <br/>Abstract: Paullones, a group of ATP-competitive 7,12-dihydroindolo [3,2-d][1]benzazepin-6(5H)-ones are well-established cyclin-dependent kinase (CDK) inhibitors with promising anti-tumoral properties. In this study, AM1 (Austin model 1) calculations have been performed on paullones and their biological activities have been explained with respect to their HOMO and LUMO energies. 9-Substituted paullones with electrophilic character show high potencies. The interaction of 9th substitutent with Phe-80 of receptor plays a key role in binding and potency. The study will help medicinal chemists to design efficient CDK inhibitiors. <br/> <br/>Page(s): 416-420 Fri, 28 Nov 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/2709 Title: Dietary cholesterol and estrogen administration elevate brain apolipoprotein E in mice by different mechanisms <br/> <br/>Authors: Srivastava, Neelam; Averna, Maurizio; Srivastava, Rai Ajit K <br/> <br/>Abstract: Apolipoprotein (apo) E plays an important role in the whole body cholesterol homeostasis. Recent studies suggest that it may also be involved in the local cholesterol transport in the brain, and influence the pathogenesis of Alzheimer’s disease (AD) by interacting with the β-amyloid protein and brain lipoprotein receptors. Since apoE expression is highest in the brain, next only to the liver and associated with the pathogenesis of AD, we hypothesized that dietary and hormonal intervention, known to regulate hepatic apoE expression may also regulate brain apoE and thereby influence local cholesterol transport. To test this hypothesis, groups of male C57BL mice were fed either regular rodent chow or high fat (HF) and high cholesterol enriched diet for 3 weeks. In a separate study, groups of male mice were administered pharmacological doses of 17-β estradiol for 5 consecutive days and sacrificed on the 6th day. As expected, HF diet elevated liver apoE mRNA and apoE synthesis. Similar to liver, brain apoE mRNA and synthesis also increased, following HF feeding. Estradiol administration increased liver apoE synthesis without affecting apoE mRNA. Interestingly, estradiol administration also increased the brain apoE synthesis, but without altering the brain apoE mRNA. These studies suggested that dietary cholesterol and estrogen administration elevated the brain apoE by different mechanisms. <br/> <br/>Page(s): 410-415 Fri, 28 Nov 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/2708 Title: Optimization of extracellular xylanase production by Sclerotinia sclerotiorum S2 using factorial design <br/> <br/>Authors: Ellouze, Olfa; Fattouch, Sami; Mestiri, Faouzi; Aniba, Mohamed Radhouen; Marzouki, Mohamed Nejib <br/> <br/>Abstract: The improvement of xylanase production by Sclerotinia sclerotiorum S2 using a liquid fermentation culture was investigated. The optimized process was divided into three basic steps: (i) evaluating xylanase inducers using different agricultural residues such as wheat bran, oat bran, orange peel and barley bran at 1% final concentration, and also filter paper. Among these, wheat bran showed the maximum activity (2.5 U/ml) at 12 days post-inoculation; (ii) for optimization, we determined the optimal concentration of inducer, the effect of phosphate anion (K₂HPO₄/KH₂PO₄) and culture aeration using a rotary shaker at 100 and 180 rpm. The optimal conditions for these three factors were determined in an experimental panel using factorial data, in which a mathematical model (Minitab software) was fitted; (iii) The optimized culture medium containing a high level of wheat bran (3%) without K₂HPO₄/KH₂PO₄ and submitted to a high agitation (180 rpm/min) increased the xylanase production from 2.5 U/ml to 4 U/ml (1.6-fold). <br/> <br/>Page(s): 404-409 Fri, 28 Nov 2008 22:58:59 GMT