http://nopr.niscair.res.in/handle/123456789/1814 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/3006 Title: Oxygen transfer conditions in the production of rainbow trout growth hormone (rtGH) by Escherichia coli <br/> <br/>Authors: Beshay, Usama <br/> <br/>Abstract: Batch cultures of Escherichia coli pRE1-rtGH 121/MZ1 were carried out at different oxygen transfer rates (OTR) enhanced by the increase of agitation and/or aeration rates. The growth kinetics of E. coli was investigated in complex medium under batch cultivation in 3 L fermenters. The agitation rates influenced both cell growth and rainbow trout growth hormone (rtGH) production in the bioreactor. It was found that increasing the agitation speed had a positive effect on the production of rtGH. Moreover, varying the airflow rate (vvm) in the fermentor under constant drive speed, cell growth and rtGH production were greatly influenced. In the production of rtGH by E. coli, high aeration rates were found to be essential for good yields of recombinant protein. Agitation speed and aeration rate could affect dissolved oxygen concentration, which in turn affected cell growth and rtGH production. Increased aeration rates induced higher rtGH production, with the highest concentration of 0.957 g/L obtained at 4.0 vvm, within 23 h. The highest volumetric productivity for rtGH of 0.042 g/L/h was obtained at both 1000 rpm and 4.0 vvm. <br/> <br/>Page(s): 225-229 Sat, 29 Mar 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/1840 Title: In vitro studies on development of gametophyte, sex-ontogeny and reproductive biology of a threatened fern, Microsorium punctatum (L.) Copel. <br/> <br/>Authors: Srivastava, Ruchi; Srivastava, Jyoti; Behera, Sandip K; Khare, P B <br/> <br/>Abstract: Studies on reproductive biology vis-a-vis conservation of threatened state of the species, Microsorium punctatum, is the major concern of the present study. The life cycle starting from spore germination, gametophyte growth and differentiation, sex ontogeny and success of sporophyte formation through intra-and inter-gametophytic selfing was scrutenised and reproductive biology of this species was completely studied. The result indicates that the species could moderately be a good colonizer, as considerable number of sporophytes was produced through selfing, but in contrast very few plants were observed in the area of its occurrence. The threatened state could be due to unfavourable conditions for gametophyte growth, mating, subsequent slow pace of sporophyte development, and both epiphytic and terrestrial habit of the species. <br/> <br/>Page(s): 266-269 Sat, 29 Mar 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/1839 Title: Rapid in vitro multiplication of Drosera burmanii Vahl.: A vulnerable and medicinally important insectivorous plant <br/> <br/>Authors: Jayaram, K; Prasad, M N V <br/> <br/>Abstract: A protocol has been stardardised for the rapid and large-scale in vitro multiplication of the vulnerable medicinal herb, Drosera burmanii Vahl. by enhanced axillary bud proliferation from shoot tip explants. In order to standardize in vitro multiplication, the effects of different strengths of Murashige and Skoog (MS) medium (1/4, 1/3, 1/2, and full strength), different percentages of sucrose (1%, 2% and 3%), various pH (3.7, 4.7, 5.7 and 6.7) and MS basal medium fortified with different concentrations of kinetin (Kn) and 6-benzylaminopurine (BAP) (0.1, 0.5, 1.0 and 2.0 mg L⁻¹ ) were tried on shoot tip explants. Maximum number of multiple shoots developed on MS medium supplemented with Kn (1.0 and 2.0 mg L⁻¹) and BAP (0.5, 1.0 and 2.0 mg L⁻¹) separately. Direct plantlet regeneration from the leaves and in vitro flowering were also observed. Rooting was best achieved on MS basal medium. This protocol could be useful for large-scale production of biomass for quercetin, plumbagin bioprospection and long term in vitro conservation. <br/> <br/>Page(s): 260-265 Sat, 29 Mar 2008 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/1838 Title: Micropropagation of a rare mangrove Bruguiera cylindrica L. towards conservation <br/> <br/>Authors: Vartak, Varsha; Shindikar, Mahesh <br/> <br/>Abstract: An in vitro propagation protocol has been developed for Bruguiera cylindrica L. (Rhizophoraceae), a rare tree mangrove found along the western coast of India. Hypocotyl segments of viviparous propagule were cultured in vitro on Murashige and Skoog’s medium (MS). Shoot and root initiation was better on modified MS (MSM) than on MS. Shoots and roots were produced from the cut surface simultaneously in 74% explants on MSM devoid of growth regulators. The explants produced several visible shoot buds on their cut surfaces, though only 1-3 shoots were developed per explant. Multiple shoots were produced by 70% explants. When cultures were initiated in monsoon, 1.6-fold increase in shoot formation and 2.4-fold increase in root formation was observed over those initiated before monsoon. During acclimatization the survival was 87%. Considering the status of this species in terms of its distribution, threats and conservation values in the study area, its in vitro propagation will compliment and strengthen the large-scale plantation activities towards the conservation or restoration of mangrove forests. <br/> <br/>Page(s): 255-259 Sat, 29 Mar 2008 22:58:59 GMT