NISCAIR Online Periodicals Repository Collection: IJBT Vol.11(2) [April 2012]

View Point : Is Bt-brinjal ready for future food?—A critical study

Thu, 29 Mar 2012 22:58:59 GMT

Title: View Point : Is Bt-brinjal ready for future food?—A critical study

Authors: Bandopadhyay, Rajib; Sinha, Purnima; Chaudhary, Bratati

Page(s): 238-240

Micropropagation of Sauropus androgynus (L.) Merr.—An important green leafy vegetable

Thu, 29 Mar 2012 22:58:59 GMT

Title: Micropropagation of Sauropus androgynus (L.) Merr.—An important green leafy vegetable

Authors: Eganathan, P; Parida, Ajay

Abstract: A micropropagation protocol has been developed for Sauropus androgynus (L.) Merr. using uninodal explants in Murashige and Skoog (MS) medium supplemented with various concentrations of cytokinins, benzyl adenine (BA) and kinetin (Kn). Shoot induction was observed in 1.0 mg/L BA and 0.1 mg/L Kn after 25 d and more number of shoots was achieved in shoot induction medium. Rooting was induced from shoots in MS medium supplemented with various concentrations of indole 3-butyric acid (IBA) and naphthalene acetic acid (NAA). All the shoots were rooted in 0.5 mg/L IBA and 0.2 mg/L NAA after 25 d. Rooted plants were transferred to soil and successfully acclimatized.

Page(s): 235-237

Homology modeling and enzyme function prediction in uncharacterized proteins of Salmonella typhi—An in silico approach

Thu, 29 Mar 2012 22:58:59 GMT

Title: Homology modeling and enzyme function prediction in uncharacterized proteins of Salmonella typhi—An in silico approach

Authors: Gore, D G; Rathod, M K; Soni, V; Rai, M M

Abstract: Salmonella typhi, a known human pathogen registering multiple drug resistance, causes majority of endemic cases in developing nations. S. typhi genome was marked with the 1220 ORFs for hypothetical proteins. Enzyme coding probability was searched in hypothetical proteins using web tools like CDD-BLAST, InterProScan, Pfam and COGs. Study sorted out 213 proteins as enzyme coding and for these proteins tertiary structures were predicted based on the homology modeling. About 89 structures were modeled for functional proteins and such a deciphered structure-function relationship could help in detail understanding of regulatory network of S. typhi and establishing new function in uncharacterized regions.

Page(s): 224-234

Genetic analysis of MHC Class II DRB gene in an endangered Jamunapari breed of goats

Thu, 29 Mar 2012 22:58:59 GMT

Title: Genetic analysis of MHC Class II DRB gene in an endangered Jamunapari breed of goats

Authors: Singh, P K; Singh, S V; Singh, M K; Saxena, V K; Singh, A V; Sohal, J S

Abstract: Present investigation was aimed to analyze the polymorphism of Class II DRB gene region of MHC, using PCR-RFLP with PstI and TaqI restriction enzymes, in endangered Jamunapari goat breed. A total of 203 Jamunapari goats (male-77 & female-126) were included in the study to know the distribution of different genotypes in the population. PstI revealed three genotypes, i.e., PP, Pp and pp, with genotype frequency as 0.054, 0.222 and 0.724, respectively. TaqI also showed TT, Tt and tt genotypes with frequencies of 0.241, 0.527 and 0.232, respectively. The frequencies of P and p alleles were 0.165 and 0.835, while frequencies of T and t alleles were 0.505 and 0.495, respectively. Marked variations were observed in the distribution of PstI and TaqI generated genotypes in the Jamunapari goat population.

Page(s): 220-223