http://nopr.niscair.res.in/handle/123456789/11108 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/11120 Title: Photoperiod influences endogenous indoleamines in cultured green alga <i>Dunaliella bardawil</i> <br/> <br/>Authors: Ramakrishna, A; Dayananda, C; Giridhar, P; Rajasekaran, T; Ravishankar, G A <br/> <br/>Abstract: Effect of light intensity and photoperiod on growth, indoleamines and carotenoid production was studied in unicellular green algae <i style="">D</i>.<i style=""> bardawil</i>. Maximum biomass and carotenoid contents were found when cultures were grown in light (intensity of 2.0 Klux) at a photoperiod of 16/8h light and dark cycle. There was a profound influence of tested photoperiod conditions of light:dark viz. 8:16, 10:14, and 12:12 hr, continuous light on indoleamines (SER and MEL) production as estimated by HPLC and confirmed by mass spectral data obtained from LC-MS-ESI studies. Serotonin level increased from 908 to 1765 pg/g fresh wt with increase in light duration and melatonin level increased from 267 to 584 pg/g fresh wt during increase in dark phase. Carotenoids production was high in continuous light than other tested conditions.<b style=""></b> <br/> <br/>Page(s): 234-240 Sat, 26 Feb 2011 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/11119 Title: Antibacterial activity of metabolite produced by <i style="">Paenibacillus polymyxa</i> strain HKA-15 against <i style="">Xanthomonas campestris</i> pv. phaseoli <br/> <br/>Authors: Mageshwaran, V; Walia, Suresh; Govindasamy, V; Annapurna, K <br/> <br/>Abstract: An antibacterial metabolite extracted from <i style="">Paenibacillus polymyxa</i> HKA-15 showed strong inhibition against <i style="">Xanthomonas campestris</i> pv. phaseoli strains CP-1-1 and M-5. Minimum inhibitory concentration (MIC) of crude extract against strains CP-1-1 and M-5 was found to be 1.7 mg/ml and 1.52 mg/ml, respectively. In UV-Vis range, the absorption peak of crude extract was maximum at 240 nm. The compound is resilience to wide range of temperature, <i>p</i>H, surfactants and organic solvents. The complete loss of activity was observed when crude metabolite was treated with pepsin (400 unit / ml). Characterization of crude metabolite suggested its hydrophobic and peptide nature. Inhibition of <i style="">Xanthomonas campestris</i> pv. phaseoli by peptide like metabolite produced by <i style="">Paenibacillus polymyxa</i> strain HKA-15 under <i style="">in vitro</i> conditions showed ecological and biotechnological potential of strain HKA-15 to control common blight disease in beans. <br/> <br/>Page(s): 229-233 Sat, 26 Feb 2011 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/11118 Title: Cholesterol-lowering effect of non-viscous soluble dietary fiber NUTRIOSE^®6 in moderately hypercholesterolemic hamsters <br/> <br/>Authors: Juhel, Christine; Tosini, Fredéric; Steib, Marlène; Wils, Daniel; Guerin-Deremaux, Laetitia; Lairon, Denis; Cara, Louis <br/> <br/>Abstract: NUTRIOSE^®6 is a new wheat starch-based low-digestible carbohydrate. This study investigated the effect of this soluble non-viscous fiber on cholesterol metabolism. Hamsters fed with 0.25% cholesterol-enriched diet (CHO) were given graded amounts of NUTRIOSE^®6, i.e. 0% (cellulose, CHO), 3% (N3), 6% (N6) or 9% (N9) (w:w). As compared to CHO diet, 9% NUTRIOSE^®6 significantly lowered plasma and LDL cholesterol by 14.5 and 23.8%, respectively. The LDL-cholesterol lowering effect was also significant with the 6% dose (-21.4%). NUTRIOSE^®6 diets prevented hepatic cholesterol accumulation (-10 to -20%) and significantly decreased bile cholesterol (-47 to -68%) and phospholipids (-30 to -45%) concentrations. The 9% NUTRIOSE^®6 diet significantly decreased the rate of dietary cholesterol absorption (-25%) and markedly stimulated faecal neutral sterol (+81%) and bile salts (+220%) excretion. No significant change in cholesterol 7-a-hydroxylase or LDL-receptor activities was observed whereas 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity was reduced by 29%. Reduced cholesterol and bile salt absorptions and lowered cholesterol synthesis are likely mechanisms underlying the cholesterol lowering effect of NUTRIOSE^®6. Results suggest the use of NUTRIOSE^®6 as a new dietary cholesterol-lowering agent that should be tested in humans as treatment and evenly prevention of mild hypercholesterolemia. <br/> <br/>Page(s): 219-228 Sat, 26 Feb 2011 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/11117 Title: Possible involvement of GABAergic mechanism in protective effect of melatonin against sleep deprivation–induced behavior modification and oxidative damage in mice <strong>[RETRACTED ARTICLE]</strong> <br/> <br/>Authors: Kumar, Anil; Singh, Anant; Kumar, Puneet <br/> <br/>Abstract: Sleep deprivation for 72 h caused anxiety like behavior, weight loss, impaired locomotor activity and oxidative damage as indicated by increase in lipid peroxidation, nitrite level and depletion of reduced glutathione and catalase activity in sleep deprived mice brain. Treatment with melatonin (5 and 10 mg/kg, ip) significantly improved locomotor activity, weight loss and antianxiety effect as compared to control (sleep deprived). Biochemically, melatonin treatment significantly restored depleted reduced glutathione, catalase activity, attenuated lipid peroxidation and nitrite level as compared to control (72 h sleep-deprived) animals. A combination of flumazenil (0.5 mg/kg, ip) and picrotoxin (0.5 mg/kg, ip) with lower dose of melatonin (5 mg/kg, ip) significantly antagonized the protective effect of melatonin. However, combination of muscimol (0.05 mg/kg, ip) with melatonin (5 mg/kg, ip) potentiated protective effect of melatonin as compared to their effect <i>per se</i>. The results suggest that melatonin may produce its protective effect by involving GABAergic system against sleep deprivation-induced anxiety like behavior and related oxidative damage. <br/> <br/>Page(s): 211-218 Sat, 26 Feb 2011 22:58:59 GMT