http://nopr.niscair.res.in/handle/123456789/7373 <b>Special Issue on New Drug Discovery from Natural Products<b> http://nopr.niscair.res.in/retrieve/35586 http://nopr.niscair.res.in/handle/123456789/7373 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/7410 Title: Online HPLC-DPPH method for antioxidant activity of <i style="">Picrorhiza kurroa</i> Royle ex Benth. and characterization of kutkoside by Ultra-Performance LC-electrospray ionization quadrupole time-of-flight mass spectrometry <br/> <br/>Authors: Bhandari, Pamita; Kumar, Neeraj; Singh, Bikram; Ahuja, Paramvir S <br/> <br/>Abstract: <i style="">Picrorhiza kurroa</i> Royle ex Benth., is widely used in the Indian systems of medicine for the treatment of various liver ailments. Since, the role of oxi­dative stress in the pathogenesis of liver injury has become generally recognized, in present study the free radical scavenging effect of <i style="">P. kurroa</i> was assessed by on-line HPLC-DPPH and colorimetric DPPH methods. The comparative study on antioxidant activity of <i style="">P. kurroa</i> extracts by both methods revealed that colorimetric method showed very less free radical scavenging effect while HPLC-DPPH method showed high activity. Further, the kutkoside, an important ingredient of a potent hepatoprotective formulation “kutkin/ picroliv” was investigated for its chemical composition by ultra-performance liquid chromatography coupled with diode array detection/electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-DAD/ESI-QTOF-MS). Kutkoside was considered to be a single compound and reported as picroside-II or kutkoside, however, present investigation illustrated that kutkoside is a mixture of iridoid glycosides namely, picroside II, picroside IV and 6-ferulloylcatalpol. <br/> <br/>Page(s): 323-328 http://nopr.niscair.res.in/handle/123456789/7409 Title: Assessment of protective role of polyherbal preparation, Livina, against anti-tubercular drug induced liver dysfunction <br/> <br/>Authors: Gulati, Kavita; Ray, Arunabha; Vijayan, V K <br/> <br/>Abstract: The present study evaluated the possible protective role of Livina (a polyherbal preparation) against anti-tubercular therapy (ATT)-induced liver dysfunction in patients of pulmonary tuberculosis. Patients were given intensive phase treatment with 4-drugs (rifampicin, INH, pyrazinamide and ethambutol) used for anti-tubercular therapy for 2 months, followed by a 4-month continuous phase treatment with 2 drugs (rifampicin and INH) under clinical advice and supervision. Both qualitative and quantitative measures of liver function were assessed, at different time intervals, before and after ATT. Analysis of data showed that the incidence of qualitative manifestations of liver dysfunction were greater in the placebo treated group as compared to the test drug group. None of the patients of either group showed clinical jaundice. Most signific changes ant were observed in the SGOT and SGPT levels in the placebo group, wherein the levels of both enzymes were higher at 4 and 8 weeks post–ATT, as compared to the respective baseline (0 week) values. When Livina (2 capsules twice daily) was given with ATT drugs, incidence of qualitative manifestation of liver dysfunction was insignificant and SGOT and SGPT levels were also significantly lower than the placebo+ATT drugs treated group. These results indicate that the test drug (Livina) was efficacious, against ATT-induced hepatic dysfunction in patients of pulmonary tuberculosis. <br/> <br/>Page(s): 318-322 http://nopr.niscair.res.in/handle/123456789/7408 Title: <img src='/image/spc_char/beta.gif' border=0 >-Amyrin from <i>Ardisia elliptica</i> is more potent than aspirin in inhibiting collagen-induced platelet aggregation <br/> <br/>Authors: Ching, Jianhong; Chua, Tung-Kian; Chin, Lee-Cheng; Lau, Aik-Jiang; Pang, Yun-Keng; Jaya, Johannes Murti; Tan, Chay-Hoon; Koh, Hwee-Ling <br/> <br/>Abstract: <i>Ardisia elliptica</i> Thunberg (Myrsinaceae) is a medicinal plant traditionally used for alleviating chest pains, treatment of fever, diarrhoea, liver poisoning and parturition complications. The objectives of the study were to investigate the effect of <i>A</i>. <i>elliptica</i> on collagen induced platelet aggregation and to isolate and purify potential antiplatelet components. Fresh <i>A. elliptica</i> leaves were extracted using methanol (70% v/v) by Soxhlet extraction and the extract was analysed for its inhibition of collagen-induced platelet aggregation. Inhibition of platelet aggregation was assessed by incubating the extracts with rabbit blood and collagen in a whole blood aggregometer and measuring the impedance. The leaf extract was found to inhibit platelet aggregation with an IC50 value of 167 <img src='/image/spc_char/micro.gif'> g/ml. Using bioassay guided fractionation, <img src='/image/spc_char/beta.gif'>-amyrin was isolated and purified. The IC50 value of <img src='/image/spc_char/beta.gif'>-amyrin was found to be 4.5 <img src='/image/spc_char/micro.gif'> g/ml (10.5 <img src='/image/spc_char/micro.gif'> <i>M</i>) while that of aspirin was found to be 11 <img src='/image/spc_char/micro.gif'> g/ml (62.7 <img src='/image/spc_char/micro.gif'> <i>M</i>), indicating that <img src='/image/spc_char/beta.gif'>-amyrin was six times as active as aspirin in inhibiting platelet aggregation. This paper is the first report that <img src='/image/spc_char/beta.gif'>-amyrin isolated from <i>A. elliptica</i> is more potent than aspirin in inhibiting collagen-induced platelet aggregation. In conclusion, <i>A. elliptica</i> leaves were found to inhibit collagen-induced platelet aggregation and one of the bioactive components responsible for the observed effect was determined to be <img src='/image/spc_char/beta.gif'>-amyrin. <br/> <br/>Page(s): 275-279 http://nopr.niscair.res.in/handle/123456789/7407 Title: Antileishmanial phenylpropanoids from <i style="">Alpinia galanga</i> (Linn.) Willd. <br/> <br/>Authors: Kaur, Amandeep; Singh, Ranvir; Dey, Chinmoy Sankar; Sharma, Shyam Sundar; Bhutani, Kamlesh K; Singh, Inder Pal <br/> <br/>Abstract: Hexane, chloroform and ethyl acetate extracts (100 µg/ml) of <i style="">Alpinia galanga</i> rhizomes exhibited significant activity <i style="">in vitro</i> against promastigotes of <i style="">L</i>. <i style="">donovani</i>. Twelve compounds namely, methyleugenol (<b>1</b>), <i style="">p</i>-coumaryl diacetate (<b>2</b>), 1'-acetoxychavicol acetate (<b>3</b>), 1'-acetoxyeugenol acetate (<b>4</b>), <i style="">trans</i>-<i style="">p</i>-acetoxycinnamyl alcohol (<b>5</b>), <i style="">trans</i>-3,4-dimethoxycinnamyl alcohol (<b>6</b>), <i style="">p</i>-hydroxybenzaldehyde (<b>7</b>), <i style="">p</i>-hydroxycinnamaldehyde (<b>8</b>), <i style="">trans</i>-<i style="">p</i>-coumaryl alcohol (<b>9</b>), galangin (<b>10</b>), <i style="">trans</i>-<i style="">p</i>-coumaric acid (<b>11</b>) and galanganol B (<b>12</b>) were isolated from these extracts. Of these, compounds <b>2</b>, <b>3</b>, <b>4</b> and <b>5</b> were found most active <i style="">in vitro</i> against promastigotes of <i style="">L</i>. <i style="">donovani</i> with IC₅₀ values of 39.3, 32.9, 18.9 and 79.9 µ<i>M</i> respectively. This is the first report of antileishmanial activity of the extracts and isolated constituents of <i style="">A</i>. <i style="">galanga</i>. <br/> <br/>Page(s): 314-317