http://nopr.niscair.res.in/handle/123456789/6433 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/6670 Title: Effect of <i style="">Aerva lanata</i> on calcium oxalate urolithiasis in rats <br/> <br/>Authors: Soundararajan, P; Mahesh, R; Ramesh, T; Begum, V Hazeena <br/> <br/>Abstract: Calcium oxalate (CaOx) stone was induced in rats using 0.75% of ethylene glycol in drinking water for 28 days. Ethylene glycol treated rats showed significant increase in the activities of oxalate synthesizing enzymes such as glycolic acid oxidase (GAO) in liver and lactate dehydrogenase (LDH) in liver and kidney. CaOx crystal deposition, as indicated by increased excretion of stone-forming constituents in urine, such as calcium, oxalate, uric acid, phosphorus and protein and decreased concentration of inhibitors, such as citrate and magnesium was observed in ethylene glycol induced urolithic rats. Histopathological studies also confirmed the deposition of CaOx crystals. Administration of <i style="">Aerva lanata</i> aqueous suspension (2g/kg body wt/dose/day for 28 days) to CaOx urolithic rats had reduced the oxalate synthesizing enzymes, diminished the markers of crystal deposition in the kidney. The results of the present study confirmed that <i style="">A. lanata</i> can be used as an curative agent for urolithiasis. <br/> <br/>Page(s): 981-986 http://nopr.niscair.res.in/handle/123456789/6669 Title: Affinity purification and partial characterization of IgM-like immunoglobulins of African catfish, <i style="">Clarias gariepinus</i> (Burchell, 1822)<b style=""></b> <br/> <br/>Authors: Rathore, Gaurav; Swaminathan, T Raja; Sood, Neeraj; Mishra, B N; Kapoor, D <br/> <br/>Abstract: IgM like macroglobulin from bovine serum albumin (BSA)-immunized African catfish <i style="">C. gariepinus</i> was purified by affinity chromatography and partially characterized. The molecular weight of this macroglobulin was 840 kDa, as estimated by gel filtration chromatography. Purified macroglobulin was analyzed using SDS-PAGE under reducing and non-reducing conditions. The molecular weight (MW) of heavy and light chain was 74.8 kDa and 27.2 kDa respectively, in presence of a reducing agent. In non-reducing SDS-PAGE, a single high MW band was observed representing tetrameric form. <br/> <br/>Page(s): 1018-1021 http://nopr.niscair.res.in/handle/123456789/6668 Title: Impact of feeding ethanolic extract of root bark of <i style="">Cananga odorata</i> (Lam) on reproductive functions in male rats <br/> <br/>Authors: Anitha, P; Indira, M <br/> <br/>Abstract: The 50% ethanolic extract of the root bark of <i style="">C. odorata</i> administered orally at the dose of 1g/kg body weight /day for 60 days resulted in decreased epididymal sperm motility and sperm count in male albino rats. Morphological abnormalities were also observed in the sperms. The testicular glycogen, the activities of 3β hydroxy steroid dehydrogenase, glucose 6-phosphate dehydrogenase, malic enzyme, sorbitol dehydrogenase in seminal vesicle, fructose in seminal plasma and serum testosterone were significantly decreased in treated group. While testicular cholesterol level, the concentration of the fecal bile acids, urinary excretion of 17 ketosteroids, the activities of 17β hydroxy steroid dehydrogenase, epididymal lactate dehydrogenase and that of testicular HMG CoA reductase were increased in treated group when compared to control. The results suggest that the ethanolic extract of <i style="">C. odorata </i>possesses the spermatotoxic effects in male albino rats. <br/> <br/>Page(s): 976-980 http://nopr.niscair.res.in/handle/123456789/6667 Title: Effects of oxidizing and reducing agents on ovine pulmonary artery responses to nitric oxide donors, sodium nitroprusside and 3-morpholino-sydnonimine <br/> <br/>Authors: Sardar, K K; Sarkar, S N; Bawankule, D U; Mishra, S K; Raviprakash, V <br/> <br/>Abstract: Nitrovasodilators-sodium nitroprusside (SNP; 10^-9-10^-4 <i style="">M</i>) and 3-morpholino-sydnonimine (SIN-1; 10^-9-10^-4 <i style="">M</i>) produced concentration-dependent relaxation of the fourth generation sheep pulmonary artery, preconstricted with 5-hydroxytryptamine (1 µ<i style="">M</i>). Oxidizing agents [oxidized glutathione (GSSG, 1 m<i style="">M</i>) and CuSO₄ (5 and 20 µ<i style="">M</i>)] and reducing agents [dithiothreitol (DTT, 0.1 m<i style="">M</i>), ascorbic acid (1 m<i style="">M</i>) and reduced glutathione (GSH, 1 m<i style="">M</i>)] caused opposite effects on nitric oxide (NO)-induced vasodilation in the artery. Ascorbic acid and GSH potentiated the NO responses, while GSSG and CuSO₄ inhibited relaxation caused by the nitrovasodilators. DTT, however, reduced the relaxant potency and efficacy of SNP and SIN-1. Pretreatment of the pulmonary artery strips with DTT (0.1 m<i style="">M</i>) inhibited SNP (10 µ<i style="">M</i>)-induced Na^+-K^+-ATPase activity, while ascorbic acid (1 m<i style="">M</i>) and GSH (1 m<i style="">M</i>) had no effect either on basal or SNP (10 µ<i style="">M</i>)-stimulated ⁸⁶Rb uptake, an index of Na^+-K^+-ATPase activity, in ovine pulmonary artery. The results suggest that reducing agents like ascorbic acid may have beneficial effect in improving the vascular function under oxidative stress. <br/> <br/>Page(s): 964-970