NISCAIR Online Periodicals Repository Collection: IJBT Vol.03(3) [July 2004]

Plant regeneration from callus cultures in Suaeda nudiflora (Wild.) Moq.

Title: Plant regeneration from callus cultures in Suaeda nudiflora (Wild.) Moq.

Authors: Singh, Aneesha; Chikara, J; Pandya, J B

Abstract: Suaeda nudiflora plants, showing relatively high salt tolerance, were used for micropropagation and generation of plantlets from callus cultures. Induction of somaclonal variability in the existing germplasm was another aim. Callus was initiated from young stem on MS medium supplemented with different concentrations of BAP and 2,4-D. Organogenesis was achieved by transferring callus on MS medium supplemented with BAP, KN and adenine. Well-grown shoots rooted easily on MS-half supplemented with IBA, IPA and NAA and 80-90% rooting was achieved. Plantlets got hardened by keeping them in hardening unit for few days. Hardened plants established very well in the nursery. Though originated from callus, these plants did not show any morphological variations and were similar to their parent donor plant.

Page(s): 454-456

Production of camptothecines from callus cultures of Nothapodytes foetida (Wight) Sleumer

Title: Production of camptothecines from callus cultures of Nothapodytes foetida (Wight) Sleumer

Authors: Sundravelan, R; Desireddy, B; Ciddi, Veeresham

Abstract: Murashige and Skoog (MS) medium supplemented with picloram (2 mg/l), N⁶- benzyladenine(BA) (1 mg/l) and gibberellic acid (GA₃) (1 mg/l) was found to be suitable for the establishment of callus cultures from leaves of Nothapodytes foetida (Wight) Sleumer. The callus upon extraction and analysis revealed the presence of a cytotoxic quinoline alkaloid, camptothecine (CPT) (2.893±2.38 mg %) and 9-methoxy camptothecine (MCPT) (0.4±0.4 mg %).

Page(s): 452-453

Direct organogenesis in Datura metel L. from in vitro and in vivo nodal explants

Title: Direct organogenesis in Datura metel L. from in vitro and in vivo nodal explants

Authors: Muthukumar, B; Arockiasamy, D I; Natarajan, E

Abstract: In vitro plant regeneration was achieved in Datura metel from nodal explants collected from both in vitro germinated seedlings and field grown plants (in vivo). The explants were cultured on MS medium with BAP (0.5-3.0 mg/l) and NAA (0.5 mg/l). The nodal explants isolated from in vivo source exhibited a greater number of healthy multiple shoots than in vitro. BAP at 3 mg/l with NAA at 0.5 mg/l was found to be optimal for regeneration of shootlets.

Page(s): 449-451

Genetic characterisation of Jaisalmeri camel using microsatellite markers

Title: Genetic characterisation of Jaisalmeri camel using microsatellite markers

Authors: Gautam, L; Mehta, S C; Gahlot, R S; Gautam, K

Abstract: Six New World Camelidae microsatellite primer pairs were used to investigate the genetic polymorphism in Jaisalmeri camel. Polymerase chain reactions were carried out for 30 unrelated camels of Jaisalmeri breed. The amplification products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. All the 6 microsatellite primer pairs were found polymorphic in Jaisalmeri camel. The number of alleles ranged from 2 to 5. The expected heterozygosity ranged from 0.32 to 0.651 and the polymorphic information content ranged from 0.268 to 0.588. The results indicated the utility of these microsatellite loci for studying genetic polymorphism in dromedary breeds.

Page(s): 457-459