http://nopr.niscair.res.in/handle/123456789/5175 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/5689 Title: Cloning of medicinal plants through tissue culture —A review <br/> <br/>Authors: Chaturvedi, H C; Jain, Madhu; Kidwai, N R <br/> <br/>Abstract: In order to have standardized formulations, the chemical constituents from plants and their parts are required to be uniform both qualitatively and quantitatively. Furthermore, an ever increasing demand of uniform medicinal plants based medicines warrants their mass cloning through plant tissue culture strategy. A good number of medicinal plants have been reported to regenerate <i style="">in vitro </i>from their various parts, but a critical evaluation of such reports reveals that only a few complete medicinal plants have been regenerated and still fewer have actually been grown in soil, while their micropropagation on a mass scale has rarely been achieved, particularly in those medicinal plants where conventional propagation is inadequate, like, the mass clonal propagation of <i style="">Dioscorea floribunda </i>leading to its successful field trials. Such facts make it imperative to document the factual position of micropropagation of medicinal plants bringing out the advancements made along with the short falls, in this important area. The present review deals with the futuristic view on the said subject restricted to higher plants. <br/> <br/>Page(s): 937-948 http://nopr.niscair.res.in/handle/123456789/5360 Title: Angiotensin converting enzyme from sheep mammary, lingual and other tissues <br/> <br/>Authors: Rao, N Mallikarjuna; Udupa, E G Padmanabha <br/> <br/>Abstract: Occurrence of angiotensin converting enzyme (ACE) in mammary gland and tongue taste epithelium was demonstrated for the first time. Six times higher ACE activity in lactating mammary gland, than non-lactating mammary gland, suggested pregnancy and lactation hormonal dependent expression of ACE in female mammals. ACE activity was highest in choroid plexus, less in spinal cord and moderate in cerebrum, medulla, cerebellum and pons. Distribution of ACE in different regions of skin, kidney and among other tissues was different. Presence of ACE in adrenal glands, pancreas, bone marrow and thyroid gland indicated functions other than blood pressure homeostasis for this enzyme. <br/> <br/>Page(s): 1003-1006 http://nopr.niscair.res.in/handle/123456789/5359 Title: Purification of elastase-like chymotrypsin from cardamom shoot and Capsule borer <br/> <br/>Authors: Josephrajkumar, A; Chakrabarty, R; Thomas, G <br/> <br/>Abstract: An elastase-like chymotrypsin was purified by aprotinin-agarose affinity chromatography from the midgut extract of cardamom shoot and capsule borer, <i style="">Conogethes punctiferalis</i>. The purified enzyme had a V_maxof 687.6 ± 22.1 nmole <i style="">p</i>NA released/min/mg protein, K_m of 0.168 ± 0.012 m<i style="">M</i> with SAAPL<i style="">p</i>NA as substrate and gave a single band on SDS-PAGE with a molecular mass of 72.1 kDa. Casein zymogram revealed one clear zone of proteolytic activity, which corresponded to the band obtained with SDS-PAGE indicating that this could be a single-polypeptide enzyme. <br/> <br/>Page(s): 998-1002 http://nopr.niscair.res.in/handle/123456789/5358 Title: <img src='/image/spc_char/alpha.gif'>₁ and <img src='/image/spc_char/alpha.gif'>₂ adrenoceptor mediated melanosome aggregatory responses <i style="">in vitro</i> in <i style="">Oreochromis mossambica </i>(Peters) melanophores <br/> <br/>Authors: Acharya, L. Shobha Kumari; Ovais, M. <br/> <br/>Abstract: Effects of specific and non-specific adrenoceptor agonists and antagonists were examined on the isolated scale melanophores of <i style="">O. mossambica</i> in physiological Ringer solution. The responses were recorded as melanophore size index. It was observed that adrenaline, nor-adrenaline, phenylpropanolamine, clonidine and phenylepherine induced melanosome aggregation in a dose-dependent manner. Denervation of the fish melanophores increased the sensitivity of the melanophores to adrenaline but not to nor-adrenaline. Phentolamine (3.55<img src='/image/spc_char/cross.gif'>10^-5 <i style="">M</i>), prazosin (2.38<img src='/image/spc_char/cross.gif'>10^-5 <i style="">M</i>) and yohimbine (2.821<img src='/image/spc_char/cross.gif'>10^-5 <i style="">M</i>) significantly inhibited the aggregatory responses of the fish melanophores to adrenaline, nor-adrenaline, clonidine and phenylepherine. The blocking effect of yohimbine was significantly higher than that of prazosin. It is concluded that the effect of adrenaline is directly mediated through the receptors and <img src='/image/spc_char/alpha.gif'>₂ adrenoceptors are predominantly involved in the aggregatory responses of this fish melanophores, while <img src='/image/spc_char/alpha.gif'>₁ adrenoceptors presence has been indicated. <br/> <br/>Page(s): 984-991