http://nopr.niscair.res.in/handle/123456789/5 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/18 Title: Effect of crude oil concentrations, temperature and pH on growth and degradation of crude oil by marine bacteria <br/> <br/>Authors: Vyas, Trupti K.; Dave, B. P. <br/> <br/>Abstract: Accidental spillage of petroleum in marine environment provokes serious damage to marine ecosystem. Microbial degradation of petroleum hydrocarbons is one of the major practice in the natural decontamination process. Out of 130 crude oil degrading bacteria isolated from Bhavnagar (Alang) coast, NW India, 12 isolates designated as TV 1 – TV 12 that formed maximum clearing zone on mineral medium were examined for their hydrocarbon degradation potential in temporal sequence of events. Among these, isolate TV 11 showed maximum growth (0.70 mg/ml) and degradation (22.8 %) on 34th day, followed by TV 4 that showed maximum growth (0.60 mg/ml) and degradation (17.0 %) and TV 3 that showed maximum growth (0.59 mg/ml) and degradation (16.2 %) on 28th day of incubation. Isolate TV 11 was identified as Marinococcus albus, TV 3 as Methylobacterium mesophilicum and TV 4 as Nocardia otitidiscaviarum. Effect of various crude oil concentrations (2 - 6 g/l), temperature (20 – 37 ºC) and pH (6 - 9) on growth and degradation revealed that 5 g/l of crude oil concentration, 30 ºC temperature for M. albus and 25 ºC for M. mesophilicum and N. otitidiscaviarum and pH 7 were optimum for both growth and degradation. Enhanced bacterial growth and degradation achieved with optimized concentrations of crude oil, temperature and pH can make their use suitable in devising an appropriate bioremediation strategy for oil contaminated sites. <br/> <br/>Page(s): 76-85 http://nopr.niscair.res.in/handle/123456789/17 Title: Determination of trace metals in seawater by ICP-MS after preconcentration and matrix separation by dithiocarbamate complexes <br/> <br/>Authors: Satyanarayanan, M; Balaram, V; Rao, T Gnaneshwar; Dasaram, B; Ramesh, S L; Mathur, Ramavati; Drolia, R K <br/> <br/>Abstract: A rapid single extraction procedure using dithiocarbamate complexing agent in methyl-isobutyl ketone (MIBK) organic phase and acid exchange back-extraction is described for the simultaneous quantitative preconcentration of Fe, Mn, V, Cr, Ni, Co, Cu, Zn, As, Mo, Cd, Pb and U in seawater followed by its determination by inductively coupled plasma mass spectrometry (ICP-MS). This method gives quantitative recoveries for all above metals at pH 4.1-6.4. A matrix matching seawater certified reference material (CRM), NASS-5, was used for calibration so as to minimize the matrix and other associated effects. Blanks and detection limits were in ng/l range. The method gives a recovery of 94-100% for 100 ml sample, facilitating the rapid and interference-free analysis of seawater samples. Excellent agreement was obtained with the certified values of another seawater CRM, CASS-4, when it was analyzed as an unknown. The major advantage of this procedure is that rapid analysis can be performed for a wide range of metals from relatively small samples. <br/> <br/>Page(s): 71-75 http://nopr.niscair.res.in/handle/123456789/16 Title: Morphometric relationship and growth of the ‘ridge back shrimp’ Solenocera choprai (Decapoda/Crustacea) from Mangalore (southwest coast of India) <br/> <br/>Authors: Dineshbabu, A.P.; Manissery, Joseph. K. <br/> <br/>Abstract: ‘Ridge back shrimp’, Solenocera choprai, caught from a depth of 70 to 100 m off Mangalore, has been contributing substantially to the commercial fishery of the coast from 1993 onwards. During 2002, the species formed > 50% of the total shrimp landing of Mangalore fisheries harbour (2746 t). Since it is a non-conventional species to commercial fishery, the detailed information about the biology and population characteristics of the species is reported for the first time. The present paper deals with the morphometric relationship like length-weight relationship and carapace length-total length relationship of the species which will be useful in studying the population dynamics of the species. The life span of S. choprai is about 30 months and males and females attain a total length of 66 mm and 83 mm at the end of 12 months, 88 mm and 109 mm at the end of 24 months respectively. The results of the study will help in formulation of strategies for the management of this resource in terms of regulation of mesh size and fishing pressure. <br/> <br/>Page(s): 65-70 http://nopr.niscair.res.in/handle/123456789/15 Title: Gastric emptying, clearance rate, feeding periodicity and food consumption of the Black Sea jelly fish, Mnemiopsis leidyi (Agassiz) <br/> <br/>Authors: Mazlum, R. Evren <br/> <br/>Abstract: The feeding physiology of Mnemiopsis leidyi fed on Artemia salina were investigated under laboratory conditions. Multifactorial experimental designs were used to study the factors affecting gastrovascular emptying in M. leidyi. A high correlation was found between number of prey ingested and digestion time. Modelling of gastric emptying time (GET,h.) of M. leidyi fed on A.salina was attempted to describe the effect of animal size, prey number, container volume and temperature on the digestion time. GET= 3.42- 0.00636 W + 0.0121 pN - 0.155 V - 0.00983 T. The clearance-rate was significantly affected by container volume and the feeding time. Multiple regression was attemped to model clearance rate: CR = 0.366 + 0.377 V - 0.197 H. Studying the feeding periodicity over a 24 h period, it was found that under laboratory condition and with readily available prey, M. leidyi feeds continuously except for the early hours of the day. Under such conditions medium size Mnemiompsis (11.36±0.38 g) consumed between 1286 and 2741 artemia daily. With the models of gastric emptying time (GET) and clearance rate (CR) presented in this study, quantitative assessment of the predatory impact of the ctenophore Mnemiopsis leidyi on the Black Sea ecosystem can be faciliated. <br/> <br/>Page(s): 59-64