http://nopr.niscair.res.in/handle/123456789/4407 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/4661 Title: Effect of dietary iron overload in rat brain: Oxidative stress, neurotransmitter level and serum metal ion in relation to neurodegenerative disorders <br/> <br/>Authors: Elseweidy, Mohamed M; El-Baky, Atef E Abd <br/> <br/>Abstract: Excess iron causes cell injury by reacting with superoxide anions (O₂) and hydrogen peroxide (H₂O₂) and producing hydroxyl radical (OH) and reactive oxygen species (ROS). In the present study, albino rats were fed with biscuits enriched with ferrous sulphate (0.3% w/w) for 10 weeks to have overload iron conditions and observed a significant decrease in serum chromium, brain serotonin and dopamine, while iron and zinc increased significantly in serum. Increasing iron level might be responsible for accelerated dopamine oxidation with subsequent quinone formation. <br/> <br/>Page(s): 855-858 http://nopr.niscair.res.in/handle/123456789/4660 Title: Na⁺ K⁺-ATPase activity in response to exogenous dehydroepiandrosterone administration in aging rat brain <br/> <br/>Authors: Taha, Asia; Mishra, Monika; Baquer, N Z; Sharma, Deepak <br/> <br/>Abstract: Influence of exogenously administered dehydroepiand-rosterone (DHEA) on the activity of Na⁺K⁺ATPase was investigated in synaptosomal fraction from cerebral cortex, cerebellum, hippocampus and medulla regions of brain of 12 and 22 months old rats. DHEA was administered daily at the dose of 30 mg/kg/body wt, intraperitonially (ip) in both the age groups of rats for 1 month. Results showed that Na⁺K⁺ATPase activity, increased in DHEA treated rats in both the age groups. In terms of per cent increase, 22 months old animals showed significant increase in Na⁺K⁺ATPase activity in the synaptosomal fraction of all the four brain regions than in 12 months old DHEA-treated rats. This showed that exogenous DHEA modulated the activity of Na⁺K⁺ATPase and also protected the age-related loss of membrane integrity and functions. It was concluded that exogenous DHEA might be beneficial in terms of neuroprotection against age-related loss of Na⁺ K⁺ ATPase mediated brain functions like learning and memory. <br/> <br/>Page(s): 852-854 http://nopr.niscair.res.in/handle/123456789/4659 Title: Regulation of urease in <i style="">Bradyrhizobium</i> colonizing green gram (<i style="">Vigna radiata</i> (L.) Wilczek) <br/> <br/>Authors: Sen, Devyani; Appunu, C; Singh, R K <br/> <br/>Abstract: In the present study attempts have been made to characterize urease expression in slow growing <i style="">Bradyrhizobium</i> strains TAL442 and MO5 which are endosymbionts of green gram (<i style="">Vigna radiata</i> (L.)Wilczek). It was found that urease activity in vegetative cells of both the strains was inducible unlike their fast growing counterparts. Mode of regulation in TAL442 was governed by presence of ammonia. Urease expression was also detectable in bacteroids of both the strains which was not influenced by presence of external nickel chloride in high concentration, a situation detrimental to the vegetative cells. <br/> <br/>Page(s): 846-851 http://nopr.niscair.res.in/handle/123456789/4658 Title: Improved and convenient method of RNA isolation from polyphenols and polysaccharide rich plant tissues <br/> <br/>Authors: Kansal, Rekha; Kuhar, Kalika; Verma, Isha; Gupta, Ram Niwas; Gupta, Vijay Kumar; Koundal, Kirpa Ram <br/> <br/>Abstract: It has been difficult to extract a good quality total RNA from the plant parts (such as seeds) which contain high levels of phenolic compounds, carbohydrates and other compounds that bind and/or co-precipitate with RNA. A simple, rapid and efficient method for isolating total RNA from polyphenols and polysaccharide rich plant tissues has been developed. Seeds of leguminosae family were chosen for the study. The good quality and high yield of total RNA was achieved with A₂₆₀/A₂₈₀ ratio of 1.9. Seeds of three different crops (<i style="">Cajanus cajan</i>, <i style="">Dolichos biflorus</i> and <i style="">Vigna mungo</i>) at different developmental stages were evaluated for total RNA extraction using standardized protocol. Seeds at 21 days after flowering (DAF) gave the best results among others (7 DAF and dry seeds). Quality of isolated RNA from all the three crops was further checked by cDNA synthesis. The extracted RNA was found suitable for further molecular applications such as reverse transcription and cDNA library construction. <br/> <br/>Page(s): 842-845