http://nopr.niscair.res.in/handle/123456789/3691 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/3803 Title: Beneficial effects of tender coconut water against isoproterenol induced toxicity on heart mitochondrial activities in rats <br/> <br/>Authors: Anurag, P; Rajamohan, T <br/> <br/>Abstract: Myocardial infarction induced by isoproterenol results in increased mitochondrial lipid peroxidation, decreased activities of carnitine acetyl transferase, acyl CoA dehydrogenase, TCA cycle enzymes and decreased rate of palmitate oxidation into CO₂ and ATP formation in the heart in rats. Isoproterenol-induced rats when fed with tender coconut water (West coast tall variety, 5-6 months age) showed improved activities of these mitochondrial enzymes and higher rate of palmitate oxidation into CO₂ and ATP production. These results indicate that intake of TCW has a significant beneficial effect on mitochondrial activities. <br/> <br/>Page(s): 278-280 http://nopr.niscair.res.in/handle/123456789/3802 Title: Purification and partial characterization of cytochrome c₅₅₂ from Halobacterium salinarium <br/> <br/>Authors: Sreeramulu, K <br/> <br/>Abstract: Cytochrome c₅₅₂ was purified to near homogenity and partially characterized from Halobacterium salinarium JWS mutant, devoid of carotenoid pigments. The purification involved the extraction of membranes with 1% Triton X-100, followed by butylagarose, DEAE-Sepharose CL6B and hydroxyapatite column chromatography. The fold of purification was 16. The purified cytochrome showed maximum absorption at 552 nm. The molecular mass determined by SDS-PAGE was found to be 14.1 kD. <br/> <br/>Page(s): 274-277 http://nopr.niscair.res.in/handle/123456789/3801 Title: Effect of terminal achiral and chiral residues on the conformational behaviour of poly Δ^ZPhe and analysis of various interactions <br/> <br/>Authors: Nandel, Fateh S; Kaur, Harpreet <br/> <br/>Abstract: Conformational properties of the peptides containing (Δ^ZPhe)6 with achiral (ΔAla, Gly) and chiral (Ala, Leu) residues at both the N- and C-terminal positions have been studied with a view to design a peptide with desired helical screw sense. In all the peptides, the lowest energy conformational state corresponds to φ = 0º and Ψ = + 90ºor – 90º or both ± 90º. These structures are characterized by rise per residue of 1.94 Å; rotation per residue of 114º and 3.12 residues per turn and are stabilized by: (i) carbonyl-carbonyl interactions with the carbonyl oxygen of ith residue and carbonyl carbon atom of the carbonyl group of ith+1 residue; and (ii) N-H…π interactions between the amino group of Δ^ZPhe and its own aromatic moiety. The Ala/Leu residues at the N-terminus further stabilized the structure, through C-H…π interactions with the farthest edge of the aromatic ring of ith+3 Δ^ZPhe residue. For peptides Ac-L-Ala/L-Leu-(Δ^ZPhe)6-NHMe, the low energy left handed helical structure (~2.5 Kcalmol-1 higher in energy) state corresponds to φ = -30º, Ψ = 120º for L-residue and φ = Ψ = 30º for Δ^ZPhe residues and is in good agreement with the X-ray crystallography results for the peptide Boc-L-Ala-(Δ^ZPhe)4-NHMe crystals grown from acetonitrile/ethanol mixture. Computational results suggest that the peptides Ac-D-Ala/D-Leu-(Δ^ZPhe)6-NHMe adopt a right handed helical structure in polar solvents with φ = 30º, Ψ = -120º for D-residues and φ = Ψ = -30º for Δ^ZPhe residues. Both in the left handed and right handed structures, the carbonyl oxygen of acetyl group is involved in 10-membered hydrogen bonded ring formation with NH of 3rd Δ^ZPhe residue whereas Δ^ZPheresidues backbone adopts a 3₁₀ helix structure. Computational results also suggest that the conformational state with φ = 0º and Ψ = 90º can be realized by keeping D-Ala or D-Leu at the C-terminal. There is hardly any effect of achiral residues Gly/ΔAla on the conformational behaviour of poly-Δ^ZPhe. <br/> <br/>Page(s): 265-273 http://nopr.niscair.res.in/handle/123456789/3800 Title: Protective role of selenium status on T₃/T₄ kinetics in rats under hyperlipidemia <br/> <br/>Authors: Dhingra, Sanjiv; Singh, Uma; Bansal, M P <br/> <br/>Abstract: The effect of high fat diet (HFD) on thyroid hormones (T₃/T₄ ) and protective role of selenium (Se) were studied in rats. Se levels in serum and liver decreased significantly, whereas glutathione peroxidase (GSH-Px) in liver and lipid levels (cholesterol and triglycerides) in serum increased after 1, 2 and 3 months of HFD feeding in comparison to controls in all the three Se status i.e. deficient (0.02 ppm), adequate (0.2 ppm) and excess (1 ppm) groups. Levels of T₃/T₄ decreased significantly on HFD feeding, as compared to respective controls in all the groups. Within the deficient group, as Se deficiency progressed, T₃/T₄ levels decreased after 2 and 3 months in comparison to 1 month. A significant increase was observed in T₃/T₄ concentration on feeding 1 ppm (excess) Se supplemented diet, in comparison to adequate group. Also, in 1 ppm Se supplemented group as the Se deposition increased i.e. after 2 and 3 months, levels of T₃/T₄ increased significantly. So, the present study indicates that Se supplementation up to 1 ppm normalizes the T₃ and T₄concentrations or regulates the hypothyroidism induced by hyperlipidemia. <br/> <br/>Page(s): 260-264