http://nopr.niscair.res.in/handle/123456789/3687 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/3743 Title: BOOK REVIEW <br/> <br/>Abstract: Mitochondria - Immo E. Scheffler, Wiley-Liss, ISBN No. 471-19422-0, John Wiley and Sons Inc., New York, 1999, pp-xi-367, Price $–not given <br/> <br/>Page(s): 66-67 http://nopr.niscair.res.in/handle/123456789/3742 Title: Purification and properties of glucose 6-phosphate dehydrogenase from turkey erythrocytes <br/> <br/>Authors: Yılmaz, Hayrullah; Beydemir, Sukru; Bakan, Ebubekir; Kufrevioglu, O Irfan <br/> <br/>Abstract: Glucose 6-phosphate dehydrogenase (G6PD) was purified from turkey erythrocytes by ammonium sulphate precipitation and followed by ADP Sepharose affinity gel chromatography. The yield was 49.71% and specific activity of the enzyme was found to be 44.16 EU/mg protein. By gel filtration the molecular mass was found to be 75 kDa. The enzyme had an optimum pH at 9.0, and optimum temperature at 50oC. Km and Vmax for NADP+ and glucose 6- phosphate (G6-P) as substrates were also determined and effects of inhibitors such as ATP, NADH and NADPH were examined. <br/> <br/>Page(s): 62-65 http://nopr.niscair.res.in/handle/123456789/3741 Title: EPR studies on Fcϒ receptor-mediated changes in lymphocyte membrane <br/> <br/>Authors: Gupta, Rajesh K; Pande, Abhay H; Hajela, Krishnan <br/> <br/>Abstract: Biophysical evidence has been presented for the interaction of human lymphocyte membrane Fc receptors with aggregated IgG by severely restricting the rotational mobility of the cell surface proteins, as well as membrane lipids. Decrease in membrane fluidity was more prominent with aggregated IgG since the multivalency of Fc regions in aggregated IgG cross-linked cell surface Fc receptor. <br/> <br/>Page(s): 59-61 http://nopr.niscair.res.in/handle/123456789/3740 Title: Computational analysis of evolutionary divergence of scorpion toxins <br/> <br/>Authors: Upadhyay, R K <br/> <br/>Abstract: Conserved and consensus sequences of several members of scorpion toxin families have been analyzed. Multiple sequence alignment define the highly conserved residues that play important structural role in formation of toxin sub-groups. Scorpion toxins have characteristic feature in signature pattern {(GA)}- K-C {(LIVM)}-X (2)- K- C- C-X-C) }, where lysine and cysteine residues are well conserved in the polypeptide. The toxins show two major groups, one with conserved active region GKCMNKGKC and second with CTPK. There is variability in position of lysine and arginine in different toxins. Most of the scorpion toxins contain six conserved cysteines involved in disulphide bonds. From the evolutionary analysis it is concluded that Leiurus is a primitive ancestral species from which probably various toxins have evolved during the evolution. <br/> <br/>Page(s): 51-58