NISCAIR Online Periodicals Repository Collection: IJBT Vol.06(2) [April 2007]

In vitro propagation of tikhur (Curcuma angustifolia Roxb.): A starch yielding plant

Title: In vitro propagation of tikhur (Curcuma angustifolia Roxb.): A starch yielding plant

Authors: Shukla, S K; Shukla, Susmita; Koche, Vijaya; Mishra, S K

Abstract: In vitro regeneration of Curcuma angustifolia Roxb. was achieved through shoot meristem culture. The shoot buds (2-3 cm long) from rhizome were inoculated on MS medium supplemented with 3.0 mg/L BAP for initiation and elongation of shoots. As a result, 1.87±0.28 shoots per explant were produced These shoots were transferred on MS medium supplemented with 3.0 mg/L BAP and 25 mg/L adenine sulfate for further shoot multiplication. About 6.9±0.69 micro-shoots per explant were produced with in 6 wk. The roots appeared from shoots on shoot establishment as well as multiplication media. The rooted plants were transferred to pots and acclimatized, which showed 83% survival with normal growth.

Page(s): 274-276

Studies on flavonoid production using in vitro cultures of Momordica charantia L.

Title: Studies on flavonoid production using in vitro cultures of Momordica charantia L.

Authors: Agarwal, Mala; Kamal, Raka

Abstract: Callus cultures and in vitro plant development of Momordica charantia L. were establishment on Murashige and Skoog’s (MS) medium using different concentrations and combinations of auxins and cytokinins. Further, qualitative and quantitative production of flavonoids in callus cultures and different in vitro developmental stages were studied by using thin layer chromatography and spectrophotometric analysis. Presence of three flavonoids was detected and the maximum amount of total flavonoid content was observed in 6-wkold callus cultures (2.90 mg/g dry wt). While in different in vitro morphogenetic stages, the maximum amount of total flavonoid content was observed in multiple shoots (2.96 mg/g dry wt).

Page(s): 277-279

Plant regeneration through somatic embryogenesis from stem and petiole explants of Indian chicory (Cichorium intybus L.)

Title: Plant regeneration through somatic embryogenesis from stem and petiole explants of Indian chicory (Cichorium intybus L.)

Authors: Abdin, M Z; Ilah, A

Abstract: An efficient protocol has been developed for rapid propagation of Indian chicory (Cichorium intybus L.) through somatic embryo development. Indirect somatic embryogenesis was induced through nodal stem and petiole explants, cultured on Murashige and Skoog (MS) medium containing different concentrations of 2,4-D, NAA or IAA. Callus was induced from both the explants after 3 wk of inoculation. Subsequently, calli were sub-cultured on MS medium containing different auxin/cytokinin ratios that influenced the intensity of embryo formation, germination and ability to regenerate plants. Somatic embryogenesis was more intensive in medium with higher concentration of Kn (1.5 mg L^-1) and lower concentration of IAA (0.5 mg L^-1) supplemented with vitamin-free casein hydrolysate (500 mg L^-1). After sub-culture, the embryoids were matured onto a fresh MS medium supplemented with Kn (1.5 mg L^-1) + IAA (0.1 mg L^-1) + IBA (1.0 mg L^-1) + CH (500 mg L^-1). The germination of embryos into shoots and less developed roots occurred on the same medium with slight change in IBB concentration (0.5 mg L^-1). Further, root formation occurred in MS medium supplemented with 1.0 mg L^-1IBA.

Page(s): 250-255

Effects of different culture media on seed germination and subsequent in vitro development of protocorms of Hygrochilus parishii (Veith & Rchb.f.) Pfitz (Orchidaceae)

Title: Effects of different culture media on seed germination and subsequent in vitro development of protocorms of Hygrochilus parishii (Veith & Rchb.f.) Pfitz (Orchidaceae)

Authors: Shadang, R; Dwivedi, Padmanabh; Hegde, S N; Ahmed, N

Abstract: Seeds of Hygrochilus parishii (Veith & Rchb.f.) Pfitz were inoculated on eight different culture media (basal) MS, MKC, V&W, I&Y and half strength of respective media. Seeds germinated within 15-20 d almost on all media with varying percentage and developed into greenish protocorm after 80-100 d. Best seed germination was observed in ½ strength MKC medium. Healthy protocorms were observed within 100 d in V&W media. Sub-cultured protocorms in all the above media supplemented with NAA, BAP, 2,4-D and IAA in a range of 0.5-2.0 mg L^-1, both individually and in combination, and additives like banana pulp (BP, 10%) and coconut milk (CM, 15%) produced well developed callus. Multiplication of protocorms was best on ½ strength MS + NAA (2.0 mg L^-1). Formation of root and leaf was early in V&W medium having CM (15%) and BP (10%) but without sucrose.

Page(s): 256-261