http://nopr.niscair.res.in/handle/123456789/1815 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/1861 Title: Efficient plant regeneration in small cardamom (Elettaria cardamomum Maton.) through somatic embryogenesis <br/> <br/>Authors: Manohari, C; Backiyarani, S; Jebasingh, T; Somanath, Archana; Usha, R <br/> <br/>Abstract: An efficient protocol for the induction of somatic embryogenesis and plant regeneration in small cardamom (Elettaria cardamomum Maton.) from the inner core region of rhizome has been established. Calli developed profusely on Murashige and Skoog (MS) medium containing 9.0 μM 2,4 D and 2.3 μM Kn. When the friable calli were cultured in MS medium containing 4.4 or 8.8 μM BAP + 0.5 μM NAA, abundant embryogenic calli were obtained. The highest frequency of embryogenic calli (68%) and plantlets (86%) were obtained from MS medium containing 4.4 μM BAP and 0.5 μM NAA. Further shoot development was observed with 13.2 μM BAP + 0.5 μM NAA. In the same medium, roots also appeared, thereby eliminating an additional step of in vitro rooting. The well-developed plants were hardened and transferred to a mist chamber in a greenhouse with 90% survival frequency. <br/> <br/>Page(s): 407-409 http://nopr.niscair.res.in/handle/123456789/1860 Title: Role of calcium ions and temperature on dextransucrase production <br/> <br/>Authors: Qader, Shah Ali Ul; Aman, Afsheen; Bano, Saeeda; Syed, Noman; Azhar, Abid <br/> <br/>Abstract: Dextransucrase [E.C. 2.4.1.5] enzyme from newly isolated strain of Leuconostoc mesenteroides PCSIR-4 showed maximum production in the fermentation medium containing 0.005% CaCl₂. The production of dextransucrase was 2.5 times higher in the medium containing calcium ions as compared to the medium without calcium ions. Also, the stability of the enzyme increased up to120 d. Maximum dextransucrase production was obtained when culture was incubated at 25C, while extracellular enzyme activity at 35C. Thermal stability of extracellular enzyme was maximum at 30C and at 50C the total enzyme loss was in 240 min. <br/> <br/>Page(s): 404-406 http://nopr.niscair.res.in/handle/123456789/1859 Title: Evaluation of genetic diversity in Mecheri sheep (Ovis aries) of Tamil Nadu using microsatellite markers <br/> <br/>Authors: Prema, S; Sivaselvam, S N; Karthickeyan, S M K <br/> <br/>Abstract: Evaluation of genetic variation was carried out using microsatellite markers in 48 Mecheri sheep of Tamil Nadu as a part of genetic characterisation and conservation. The number of observed alleles ranged from 3 to 8 with a mean of 5 across all loci. The size of alleles ranged from 74 to 224 bp. The frequency of alleles ranged from 0.0208 to 0.6250. The polymorphism information content (PIC) values varied from 0.52 to 0.79 with a mean of 0.66. The population was not in Hardy-Weinberg equilibrium. The overall mean expected heterozygosity was 0.71. The inbreeding estimate within-population was 0.004, indicating excess of heterozygotes in the population of Mecheri sheep. The panel of microsatellites used was highly informative for molecular characterisation and could be used for exploitation of genetic diversity of the related breeds for conservation. <br/> <br/>Page(s): 401-403 http://nopr.niscair.res.in/handle/123456789/1858 Title: Polymorphism in DRB3 exon 2 by PCR-RFLP and its association with mastitis in Nili-Ravi breed <br/> <br/>Authors: Kumar, Sudhir; Sangwan, M L <br/> <br/>Abstract: The present investigation was undertaken to study the genetic polymorphism in exon 2 of DRB3 gene in Nili-Ravi (n=25) buffalo breed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and its association with mastitis. The gDNA was isolated from whole blood samples. When 304 bp PCR product of exon 2 of DRB3 gene was digested with Rsa1, 11 genotypes, viz., b/b, c/c, f/f, o/o, s/s, f/o, b/f, b/o, o/s, b/l and l/s, with frequency range 0.04-0.16 and 6 alleles, viz., b, c, f, l, o and s with frequency range 0.08-0.26 were observed. HaeIII detected 6 genotypes, viz., a/a, e/e, d/d, a/b, b/d and b/e with frequency range 0.04-0.28 and four alleles, viz., a, b, d and e with frequency range 0.08-0.6. However, Pst1 revealed 5 genotypes, viz., y/y, z/z, x/y, x/z, s/z and y/z with frequency range 0.08-0.32 and four alleles, viz., x, y, z, and s with frequency range 0.04-0.42. These results revealed that exon 2 of DRB3 gene was highly polymorphic in Nili-Ravi breed. Certain genotypes (c/c, f/l, b/f, o/o, l/s, a/a and e/e) were observed only in healthy animals, while others (b/f, b/o, f/o and y/z) in mastitis cases. Result to be tested on large sample size before its practical application. <br/> <br/>Page(s): 398-400