NISCAIR Online Periodicals Repository Collection: IJBT Vol.11(3) [July 2012]

View Point: Should microarrays technology be included in medical curriculum?

Title: View Point: Should microarrays technology be included in medical curriculum?

Authors: Barik, Mayadhar; Das, Rashmi Ranjan

Page(s): 355-356

Indirect organogenesis from nodal explants of Coccinia grandis (L.) Voigt

Title: Indirect organogenesis from nodal explants of Coccinia grandis (L.) Voigt

Authors: Thiripurasundari, U; Rao, M V

Abstract: The present study reports an efficient protocol for indirect shoot organogenesis and plantlets regeneration of Coccinia grandis (L.) Voigt from nodal explants. The maximum frequency of organogenic callus induction was observed in MS medium supplemented with 0.1 mg/L 1-naphthaleneacetic acid (NAA) and 1.0 mg/L N⁶-benzyladenine (BA). Multiple shoot induction was achieved from the surface of callus on regeneration medium [MS nutrients supplemented with NAA, BA and various concentrations of kinetin (KIN) 0.1-2.0 mg/L]. The highest shoot multiplication (90%) with the increased numbers of shoots (8.7±1.8) and the shoot mean length (8.8±0.4 cm) was achieved on MS medium with 0.1 mg/L NAA, 1.0 mg/L BA and 0.5 mg/L KIN. The regenerated shoots were rooted in vitro on MS medium containing 0.1 mg/L IBA. Plantlets with well developed shoot and root systems were successfully acclimatized (83.5%) and exhibited normal morphology and growth characteristics.

Page(s): 352-354

Somatic embryogenesis in Jatropha curcus L. using cotyledonary leaves

Title: Somatic embryogenesis in Jatropha curcus L. using cotyledonary leaves

Authors: Saxena, Sonal; Sharma, Amit; Sardana, Jyoti; Sharma, Madan Mohan; Batra, Amla

Abstract: An efficient protocol has been developed for high frequency plant regeneration through somatic embryogenesis in the biodiesel plant, Jatropha curcus. Cotyledonary leaves were used for the development of the embryogenic callus on MS medium with 0.2 mg/L indole-3-acetic acid (IAA), which developed within 3-4 wk. Later, somatic embryos developed on MS medium with IAA (0.2 mg/L)along with 6-benzyl aminopurine (BAP; 1.5 mg/L). Fist, globular embryo clumps were seen within 3-4 wk and then they got transformed into heart, torpedo and cotyledonary shaped embryos on the same medium after 6 wk. On further subculture on the same medium, embryos germinated with shoot and root primordia, which ultimately gave rise to complete plantlets within 4-5 wk. The pantlets were hardened and transferred to the soil. Around 50% plantlets survived in the soil.

Page(s): 348-351

In vitro shoot multiplication from nodal explants of Boucerosia diffusa Wight—An endemic medicinal plant

Title: In vitro shoot multiplication from nodal explants of Boucerosia diffusa Wight—An endemic medicinal plant

Authors: Ramadevi, T; Ugraiah, A; Pullaiah, T

Abstract: In vitro propagation of Boucerosia diffusa Wight (Apocynaceae), an endemic medicinal plant of Western Ghats, (Tamil Nadu, India) was carried out. Nodal explants were cultured on MS medium supplemented with cytokinins [benzyl aminopurine (BAP), kinetin (Kn), thidiazuron (TDZ) & zeatin] either individually or in combinations. Shoot buds proliferated on MS medium+BAP (8.87 µM) and repeated subculture on MS+BAP (8.87 µM)+TDZ (2.27 µM) promoted the shoot multiplication. The regenerated shoots were then rooted on MS+NAA (2.69 µM). The micropropagated planlets were hardened with the survival success rate of 56%. The developed micropropagation protocol would help ex situ conservation of this endemic species.

Page(s): 344-347