http://nopr.niscair.res.in/handle/123456789/13714 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/13739 Title: Safety and immunogenicity of <i style="">Brucella</i> <i style="">abortus </i>strain RB51 vaccine in cross bred cattle calves in India <br/> <br/>Authors: Singh, Rashmi; Basera, Sanjay Singh; Tewari, Kamal; Yadav, Shweta; Joshi, Sumit; Singh, Brajesh; Mukherjee, Falguni <br/> <br/>Abstract: Safety and immunogenicity of <i style="">Brucella abortus</i> RB51 vaccine has been evaluated in an organised dairy farm in India. All the cattle (n=29) vaccinated with strain RB51 ‘responded’ to the vaccine as demonstrated by iELISA using acetone killed strain RB51 antigen. The percentage responders at day 35, 60 and 90 post vaccination were 100%, 95% and 20%, respectively. Strain RB51 was able to elicit a good IFN-<img src='/image/spc_char/gamma2.gif' border=0> response from vaccinated animals. The post-vaccination time point analysis indicated that the cumulative IFN-<img src='/image/spc_char/gamma2.gif' border=0> response of whole blood from vaccinates stimulated with heat killed RB51 antigen was elicited in 80% of calves at 60 days post vaccination. Absence of strain RB51 in the secretions and excretion and lack of local or systemic reaction indicated the safety of the vaccine. <br/> <br/>Page(s): 239-242 http://nopr.niscair.res.in/handle/123456789/13738 Title: Mortality and testicular derangements in red flour beetles, <i style="">Tribolium castaneum</i> (Herbst) exposed to hen’s egg white proteins <br/> <br/>Authors: Parshad, Ranjit K; Kansal, Megha <br/> <br/>Abstract: Red flour beetle (<i>T. castaneum</i>) is a major pest of stored grains and is known for its adaptability to all classes of insecticides. The present study was carried out to determine the insecticidal potential of egg white proteins to manage beetle population. Protein samples obtained through salt fractionation were lyophilized and were used separately and simultaneously in different concentrations by adding them to wheat flour and milk powder. The results indicated that the mortality rate of the adult beetles was dependent on the type of treatment, concentration of protein samples and duration of feeding. In multiple-choice feeding trials beetles showed their movement towards the control section as the concentration of treatment increases. Marked abnormalities were observed in appearance and dimensions of the testes which indicated that the egg white proteins caused considerable effect on the process of spermatogenesis and sperm functions. SEM study revealed the formation of deep wrinkles and folds on the testicular surface of the testes of beetles fed on treated diets, points towards the depletion of internal cellular material. The results suggest that egg white protein affects the survival and cause subsequent derangements in the testis of red flour beetle. <br/> <br/>Page(s): 232-238 http://nopr.niscair.res.in/handle/123456789/13737 Title: Effect of consumption of fatty acids, calcium, Vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats <br/> <br/>Authors: Naghii, M R; Ebrahimpour, Y; Darvishi, P; Ghanizadeh, G; Mofid, M; Torkaman, G; Asgari, A R; Hedayati, M <br/> <br/>Abstract: The consumption of fatty acids, nutrients, and regular physical activity, individually influence bone mechanical properties in rats. To investigate their effects in combination, male rats were divided into the seven groups<b>:</b> G1: regular food and drinking water; G2: same as Gr.1 + physical activity (Whole body vibration; WBV); G3: same as Gr.2 + Calcium, Vit. D, Boron; G4: same as Gr.3 + canola oil; G5: same as Gr.3 + sunflower oil; G6: same as Gr.3 + mix of sunflower oil and canola oil; and G7: same as Gr.3 + coconut oil; and treated for 8 weeks. Analysis between the control with the groups 2 and 3 revealed that vibration in the G2 increased the body weight (P= 0.04), with no other major difference in plasma and bone indices. Comparison between the control with the G4-G7 (the oil groups) revealed that the rats in the G5 had a lower body weight (15 % less) and a significant increase in plasma levels of Estradiol in the G7 was noted. In addition, levels of Testosterone in the G4 and G7, and Free Testosterone in the G7 had a remarkable increase. Similar trend was observed for plasma levels of Vit. D in the G4 and G5. The stiffness and the breaking strength of the femur in the G7, and the breaking strength of the lumbar in the G7 compared to the control and the G4 and G5 was significantly higher and tended to increase in comparison to the G6. Better and stronger measurements observed for coconut oil is warranted to further study its effect on biomechanical properties of bones. <br/> <br/>Page(s): 223-231 http://nopr.niscair.res.in/handle/123456789/13736 Title: Effect of rosuvastatin on obesity-induced cardiac oxidative stress in Wistar rats—A preliminary study <br/> <br/>Authors: Ansari, Javed A; Bhandari, Uma; Pillai, K K; Haque, S E <br/> <br/>Abstract: The prevalence of obesity has been rising alarmingly and it has now become a global concern causing an enormous economic burden on the health care system. Obesity is generally linked to complications in lipid metabolism and oxidative stress. The aim of the present study was to investigate the effect of rosuvastatin (10 mg/kg, po) on obesity-induced oxidative stress in high fat-fed Wistar rats. Oral administration of rosuvastatin (10 mg/kg) for 21 days along with high fat diet brought about significant elevation in serum high density lipoprotein and cardiac antioxidant enzymes levels (superoxide dismutase, catalase, glutathione, glutathione peroxidase, glutathione peroxidase-, glutathione reductase-and glutathione-S-transferase)<b style=""> </b>while decreasing in serum lactate dehydrogenase, apolipoprotein-B, lipids (triglycerides, total cholesterol, low density lipoprotein-cholesterol, very low density lipoprotein-cholesterol and atherogenic index) and cardiac thiobarbituric acid reactive substances levels. The results were comparable with orlistat, a standard antiobesity drug. These preliminary results for the first time demonstrate that administration of rosuvastatin can be beneficial for the suppression of obesity-induced oxidative stress and dyslipidemia in high fat-fed Wistar rats. <br/> <br/>Page(s): 216-222