http://nopr.niscair.res.in/handle/123456789/10980 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/11010 Title: Induction of systemic resistance in different varieties of <i style="">Solanum tuberosum </i>by pure and crude elicitor treatment <br/> <br/>Authors: Bariya, Himanshu S; Thakkar, Vasudev R; Thakkar, Amit N; Subramanian, R B <br/> <br/>Abstract: A 10 kD elicitor protein (infestin) produced by <i style="">Phytopthora infestans </i>was purified and its efficacy for induction of systemic resistance in resistant and susceptible varieties of <i style="">Solanum tuberosum </i>was studied. Culture filtrates from <i style="">P. infestans </i>with and without purified elicitor (infestin) were used as elicitors to understand the effect of purified elicitor (infestin) on development of systemic resistance. Culture filtrate and purified elicitor (infestin) were found to induce hypersensitive reaction on the leaves of resistant varieties, but not on susceptible varieties after 48 h. Culture filtrate devoid of purified elicitor (infestin) did not induce any necrotic spots even on resistant variety. Purified elicitor (infestin) was found to induce glucose oxidase, NADPH oxidase, superoxide dismutase, glutathione reductase, catalase and peroxidase enzymes in resistant <i style="">S. tuberosum </i>plants, however the induction of these enzymes was low in susceptible varieties. The oxidative enzymes were found to induce earlier than antioxidative enzymes and there was negative correlation between these two groups of enzymes. Levels of salicylic acid, phenylalanine ammonia lyase (PAL), <img src='/image/spc_char/beta.gif' border=0>-1, 3 glucanase and chitinase activities were also found higher in resistant than in susceptible varieties. It was observed that purified elicitor (infestin) was superior to crude culture filtrate, but was not capable of inducing systemic resistance in susceptible varieties. <br/> <br/>Page(s): 151-162 http://nopr.niscair.res.in/handle/123456789/11009 Title: Acute and sub-acute toxicity of ethanolic extract of <i>Canthium mannii </i>Hiern stem bark on <i>Mus musculus</i> <br/> <br/>Authors: Poné, J Wabo; Mbida, Mpoame; Bilong, C F Bilong <br/> <br/>Abstract: Acute and sub-acute toxicity of ethanolic extract (ETE) of <i>C. mannii </i>was assessed on white mice (<i>Mus musculus).</i> After 48 h of extract administration, no death was registered. It was deduced that the LD₅₀ was indisputably higher than 16 g/kg body weight. The sub-acute toxicity test was based on the daily administration of three doses of ETE (300, 600 and 1200 mg/kg body weight) for four weeks; 1% DMSO served as negative control. As for the first experiment, no sign of toxicity was registered. Conversely, the sub acute doses stimulated and increased the weight-rate of mice after 7 days of treatment. Except for the spleen weight, the doses administrated did not modify the weight index. It was observed that, sub-acute doses induced and increased (a) the food (particularly) and water consumption according to time and (b) the number of red and white blood cells. It was thought that, ETE can stimulate the haematopoietic function. Finally, no time variation of the activity of alanine aminotransferase and aspartate aminotransferase enzyme was observed in the serum of euthanized mice. The results showed the innocuity of ETE of <i>C. mannii </i>and<i> </i>thus validated his utilization in cameroonian traditional pharmacopoea. <br/> <br/>Page(s): 146-150 http://nopr.niscair.res.in/handle/123456789/11008 Title: Development and <i style="">in vitro</i> characterization of a bivalent DNA containing HN and F genes of velogenic Newcastle disease virus <br/> <br/>Authors: Chaturvedi, Uttara; Kalim, Shahina; Desai, G; Ratta, Barkha; Kumar, Rajiv; Ravindra, P V; Kumar, Sudesh; Dash, B B; Tiwari, Sangeeta; Sahoo, A P; Tiwari, Ashok K <br/> <br/>Abstract: Newcastle disease (ND) is highly contagious, economically important viral disease affecting most of avian species worldwide. Newcastle disease virus (NDV) has single stranded negative sense RNA genome which encodes for six structural and two non-structural proteins. Envelope glycoproteins i.e. hemagglutinin-neuraminidase (HN) and the fusion (F), elicit protective immune response. In this study, HN and F genes of velogenic (virulent) strain were amplified and cloned at multiple cloning sites A and B, respectively into pIRES bicistronic vector for use as bivalent DNA vaccine against ND. The recombinant plasmid was characterized for its orientation by restriction enzyme digestion and PCR. Expression of HN and F genes was assessed in transfected Vero cells at RNA level using RT-PCR in total RNA as well as protein level using IFAT, IPT and western blot using NDV specific antiserum. All these experiments confirmed that HN and F genes cloned in recombinant pIRES.nd.hn.f are functionally active. The recombinant construct is being evaluated as DNA vaccine against ND <br/> <br/>Page(s): 140-145 http://nopr.niscair.res.in/handle/123456789/11007 Title: Ameliorative role of atorvastatin on methionine-induced hyperhomocysteinemia and hematological changes in albino rats <br/> <br/>Authors: Bhandari, Uma; Pathan, Rahila Ahmad; Kumar, Vinay; Khanna, Naresh <br/> <br/>Abstract: Methionine (1g/kg, po) administration to pathogenic control rats for 30 days significantly increased the levels of homocysteine, total cholesterol (TC), low density lipoprotein (LDL-C), very low density lipoprotein (VLDL-C) and triglycerides (TGs) and decreased the levels of high density lipoprotein (HDL-C) in serum. Hematological observations of the peripheral blood smears of pathogenic rats fed with methionine also showed crenation of RBCs cell membrane and significant increase in total leukocyte count, differential leukocyte count and platelet counts with significant decrease in the mean hemoglobin levels as compared to vehicle control rats. Administration of atorvastatin (0.2 mg/kg/po) to hyperhomocysteinemic rats significantly decreased the levels of homocysteine, TC, TGs, LDL-C and VLDL-C and increased the levels of HDL-C in serum. The present results provide clear evidence that oral treatment with atorvastatin exhibit homocysteine and lipid lowering activity and also reversal of hematological changes induced by methionine in albino rats. <br/> <br/>Page(s): 132-139