http://nopr.niscpr.res.in/handle/123456789/38 2022-10-26T12:06:39Z 2022-10-26T12:06:39Z Thakur, Julie Abbas, N.S. Bhardwaj, Sujata Kaula, Babeeta C http://nopr.niscpr.res.in/handle/123456789/60526 2022-09-21T09:59:03Z 2022-09-01T00:00:00Z

Title: Biotechnological tools in the propagation and conservation of threatened species: An overview Authors: Thakur, Julie; Abbas, N.S.; Bhardwaj, Sujata; Kaula, Babeeta C Abstract: Plant diversity is crucial for the balanced functioning of the environment, as humans are highly dependent on them since plants provide sustenance and services like regulation of climate and protection from natural hazards. At present, 20,360 plant species are threatened (critically endangered, endangered and vulnerable), 165 species are extinct and extinct in the wild, due to several factors as per International Union for Conservation of Nature (IUCN) data and their conservation is of utmost importance. The in vitro methodology has become one of the promising tools of biotechnology in the conservation of threatened plants. The technique ensures propagation of taxa with limited explant source, seed dormancy, self-incompatibility, inbreeding depression, etc. resulting in lower seed production. In vitro methods along with the ex situ and in situ methods have been extensively used in the conservation of plants. This review highlights a compilation of several biotechnological tools i.e., micropropagation, somatic embryogenesis, callus induction, organogenesis, cryopreservation, micrografting, employed in the conservation of over a hundred threatened species. Furthermore, the present review sheds light on the importance of genetic homogeneity assessment based on frequently used molecular markers amongst in vitro raised and the donor mother plants. The review will help other researchers to employ various tissue culture methods in conservation programs of threatened and rare plants. Page(s): 217-235

2022-09-01T00:00:00Z Gupta, Vandana Nayak, Anju Swamy, Madhu Verma, Rupesh Pandey, Megha http://nopr.niscpr.res.in/handle/123456789/60525 2022-09-21T09:56:09Z 2022-09-01T00:00:00Z

Title: Molecular detection and phylogenetic analysis of lumpy skin disease virus from outbreak in Madhya Pradesh, India 2020 Authors: Gupta, Vandana; Nayak, Anju; Swamy, Madhu; Verma, Rupesh; Pandey, Megha Abstract: Lumpy skin disease (LSD) is an emerging, economically important infectious viral disease of cattle caused by Capripoxvirus. An outbreak of LSD has been reported in various states including Madhya Pradesh, India. This study was undertaken to do molecular characterization of the lumpy skin disease virus (LSDV) and to study its phylogenetic relationship with the sequences available in National Centre for Biotechnology Information (NCBI) database. A total of 6 blood samples and 2 tissue skin nodule samples were collected from cattle (aged from 6 months to 6 years) biopsies from Bamhnoda & Sagda Jhapani village of Jabalpur District of Madhya Pradesh, India and were screened for LSDV by PCR. The samples were subjected for amplification of P32 and fusion genes. Two tissue and three blood samples were positive for P32 gene. Partial P32 and fusion genes were amplified and sequenced and subjected for phylogenetic analysis by MEGA6. The phylogenetic analysis based on partial P32 gene and fusion gene showed close proximity with isolates from Egypt, Saudi Arabia and China. Page(s): 236-241

2022-09-01T00:00:00Z Ray, Mausumi Kundu, Pradyut Adhikari, Sunita http://nopr.niscpr.res.in/handle/123456789/60524 2022-09-21T09:53:42Z 2022-09-01T00:00:00Z

Title: Microbial production of L-asparaginase by an isolated Bacillus paramycoides MRS4 strain and optimization of process parameters Authors: Ray, Mausumi; Kundu, Pradyut; Adhikari, Sunita Abstract: L-asparaginase, a hydrolytic enzyme which breakdowns L-asparagine, a non-essential aminoacid to L-aspartic acid with the release of ammonia. In this research work various sources like soil, cereals: corn and oat, pulses: soya bean, vegetable: potato were used for isolation of L-asparaginase producing bacterial strain. It was found that among the all isolated bacterial strain, a strain isolated from soya bean produced maximum amount of enzyme. The isolated strain MRS4 was found having significant similarity with Bacillus paramycoides strain MCCC 1A04098 based on nucleotide homology and phylogenetic analysis. After optimization of various process parameters, it was found the strain was able to produce almost 118 IU/ml of enzyme in the selected medium when inoculated with 1% inoculums of 20 hr age in 50 ml medium taken in a 250 ml Erlenmeyer flask and incubated at 35C, pH 7, agitation speed of 150 rpm, aeration rate of 0.25 L/min for 24 hrs. Page(s): 242-251

2022-09-01T00:00:00Z Reis, Sávio Pinho dos Barros, Nicolle Louise Ferreira Siqueira, Andrei Silva Souza, Cláudia Regina Batista de http://nopr.niscpr.res.in/handle/123456789/60523 2022-09-21T09:51:15Z 2022-09-01T00:00:00Z

Title: A copper-zinc superoxide dismutase of Piper nigrum: Molecular cloning, recombinant expression, in vitro activity and molecular modeling Authors: Reis, Sávio Pinho dos; Barros, Nicolle Louise Ferreira; Siqueira, Andrei Silva; Souza, Cláudia Regina Batista de Abstract: Superoxide dismutase (SOD) plays crucial role controlling oxidative burst produced during interactions of plants with biotic factors. Previous studies identified a partial cDNA sequence coding for SOD in black pepper (Piper nigrum L.) infected by Fusarium solani f. sp. piperis, causal agent of rot root disease. Here, our main aim was to isolate this full-length cDNA sequence and characterize the P. nigrum SOD, named PnCuZnSOD. The 738-bp full-length cDNA contains a 459-bp open reading frame (ORF) encoding a deduced protein with 152 amino acid residues, predicted to be a cytosolic protein with molecular weight and isoelectric point of 15 kDa and 5.27, respectively. Comparative sequence analysis revealed high identity between the deduced PnCuZnSOD and superoxide dismutase sequences from GenBank database. In addition, putative conserved motifs, copper- and zinc-binding domains and cysteine residues involved in disulfide bond were identified. Furthermore, molecular modeling analyzes generated the three-dimensional structure of PnCuZnSOD protein. The recombinant PnCuZnSOD was produced by heterologous expression in bacterial cells, followed by the His-tagged protein purification and enzymatic activity evaluation. The effects of heat and hydrogen peroxide on the PnCuZnSOD activity were evaluated. The results indicated PnCuZnSOD as a probable active protein contributing to reactive oxygen species (ROS) homeostasis in the cytosol of P. nigrum cells. Page(s): 252-261

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